PNK Antibody (F-5): sc-166153

PNK Antibody (F-5) is a mouse monoclonal IgG1 antibody that detects polynucleotide kinase (PNK) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PNK is a crucial nuclear protein encoded by the PNKP gene, playing a vital role in DNA repair mechanisms, particularly in response to damage from radiation or oxidative stress. Anti-PNK antibody (F-5) possesses both 3′-phosphatase and kinase activities, allowing PNK to catalyze the phosphorylation of DNA at hydroxy termini while also dephosphorylating 3′-phosphate termini, which is essential for maintaining genomic integrity. The structural features of PNK, including its 3′-phosphatase domain that shares similarities with L-2-haloacid dehalogenases and its ATP binding site, are significant as they facilitate its dual enzymatic functions, thereby contributing to effective DNA repair processes. PNK is predominantly expressed in the testis, pancreas, spleen, kidney, and heart, highlighting its importance in various physiological contexts. PNK monoclonal antibody (F-5) is an invaluable tool for researchers studying DNA repair pathways and the cellular response to genotoxic stress.

The preferred PNK antibody is PNK Antibody (F-3): sc-271505.