PNK Antibody (F-3): sc-271505

PNK Antibody (F-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects PNK protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). PNK (F-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The bifunctional polynucleotide phosphatase/kinase (PNK), also known as polynucleotide kinase-3′-phosphatase, plays a crucial role in DNA repair mechanisms, particularly in response to damage from radiation or oxidative stress. PNK, encoded by the PNKP gene, features a 3′-phosphatase domain that shares similarities with L-2-haloacid dehalogenases and possesses an ATP binding site, which is essential for enzymatic activity. PNK is predominantly located in the nucleus, where PNK catalyzes the phosphorylation of DNA at hydroxy termini while also having the ability to dephosphorylate 3′-phosphate termini. PNK expression is notably high in tissues such as the testis, pancreas, spleen, kidney, and heart, highlighting its importance in cellular processes related to DNA integrity and repair.

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PNK Antibody (F-3) References:

1. Molecular cloning of the human gene, PNKP, encoding a polynucleotide kinase 3'-phosphatase and evidence for its role in repair of DNA strand breaks caused by oxidative damage.  |  Jilani, A., et al. 1999. J Biol Chem. 274: 24176-86. PMID: 10446192
2. Molecular characterization of a human DNA kinase.  |  Karimi-Busheri, F., et al. 1999. J Biol Chem. 274: 24187-94. PMID: 10446193
3. Production, characterization, and epitope mapping of monoclonal antibodies against human polydeoxyribonucleotide kinase.  |  Fanta, M., et al. 2001. Hybridoma. 20: 237-42. PMID: 11604109
4. Pnk1, a DNA kinase/phosphatase required for normal response to DNA damage by gamma-radiation or camptothecin in Schizosaccharomyces pombe.  |  Meijer, M., et al. 2002. J Biol Chem. 277: 4050-5. PMID: 11729194
5. Association of XRCC1 and tyrosyl DNA phosphodiesterase (Tdp1) for the repair of topoisomerase I-mediated DNA lesions.  |  Plo, I., et al. 2003. DNA Repair (Amst). 2: 1087-100. PMID: 13679147
6. Characterization of polynucleotide kinase/phosphatase enzymes from Mycobacteriophages omega and Cjw1 and vibriophage KVP40.  |  Zhu, H., et al. 2004. J Biol Chem. 279: 26358-69. PMID: 15056675
7. The molecular architecture of the mammalian DNA repair enzyme, polynucleotide kinase.  |  Bernstein, NK., et al. 2005. Mol Cell. 17: 657-70. PMID: 15749016
8. An end-healing enzyme from Clostridium thermocellum with 5' kinase, 2',3' phosphatase, and adenylyltransferase activities.  |  Martins, A. and Shuman, S. 2005. RNA. 11: 1271-80. PMID: 15987807
9. Mechanism of the phosphatase component of Clostridium thermocellum polynucleotide kinase-phosphatase.  |  Keppetipola, N. and Shuman, S. 2006. RNA. 12: 73-82. PMID: 16301605

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PNK Antibody (F-3) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-PNK Antibody (F-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PNK Antibody (F-3) Specifications

Alternate Product Name: PNK

Product Clone Name: F-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 457-477

Epitope Target: PNK

Epitope Species: human

Product Reactivity/Detection: PNK

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for PNK Antibody (F-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin