PNK Antibody (E-9): sc-271135

PNK Antibody (E-9) is a mouse monoclonal IgG2a antibody that detects polynucleotide kinase (PNK) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). PNK is a crucial nuclear protein encoded by the PNKP gene, playing a vital role in DNA repair mechanisms, particularly in response to damage from radiation or oxidative stress. Anti-PNK antibody (E-9) recognizes a protein that possesses both 3′-phosphatase and kinase activities, allowing PNK to catalyze the phosphorylation of DNA at hydroxy termini while also dephosphorylating 3′-phosphate termini, which is essential for maintaining genomic integrity. The structural features of PNK, including its 3′-phosphatase domain and ATP binding site, are significant as they facilitate its dual enzymatic functions, thereby contributing to the repair of single-strand breaks in DNA. PNK is highly expressed in tissues such as the testis, pancreas, spleen, kidney, and heart, indicating its importance in cellular processes across various organ systems. PNK monoclonal antibody (E-9) serves as an invaluable tool for researchers studying DNA repair pathways and the cellular response to genotoxic stress.

The preferred PNK antibody is PNK Antibody (F-3): sc-271505.