PNK Antibody (B-5): sc-365724

PNK Antibody (B-5) is a mouse monoclonal IgG1 antibody that detects polynucleotide kinase (PNK) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PNK is a crucial nuclear protein encoded by the PNKP gene, playing a vital role in DNA repair mechanisms, particularly in response to damage from radiation or oxidative stress. Anti-PNK antibody (B-5) recognizes a protein that possesses both 3′-phosphatase and kinase activities, allowing PNK to catalyze the phosphorylation of DNA at hydroxy termini while also dephosphorylating 3′-phosphate termini, which is essential for maintaining genomic integrity. The structural features of PNK, including its 3′-phosphatase domain that shares similarities with L-2-haloacid dehalogenases and its ATP binding site, are significant as they facilitate its dual enzymatic functions, thereby contributing to effective DNA repair processes. PNK exhibits the highest expression levels in tissues such as the testis, pancreas, spleen, kidney, and heart, underscoring its importance in cellular functions across various biological systems. PNK monoclonal antibody (B-5) serves as an invaluable tool for researchers studying DNA repair pathways and the implications of PNK in cellular responses to DNA damage.

The preferred PNK antibody is PNK Antibody (F-3): sc-271505.