PNK Antibody (A-4): sc-271525

PNK Antibody (A-4) is a mouse monoclonal IgG1 antibody that detects polynucleotide kinase (PNK) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PNK is a crucial nuclear protein encoded by the PNKP gene, playing a vital role in the DNA repair process, particularly in response to damage from radiation or oxidative stress. This protein possesses both 3′-phosphatase and kinase activities, allowing PNK to catalyze the phosphorylation of DNA at hydroxy termini while also dephosphorylating 3′-phosphate termini, which is essential for maintaining genomic integrity. PNK′s ability to facilitate the repair of damaged DNA underscores PNK′s importance in cellular survival and function, especially in tissues with high turnover rates such as the testis, pancreas, spleen, kidney, and heart, where PNK is expressed at elevated levels. Anti-PNK antibody (A-4) is an invaluable tool for researchers studying DNA repair mechanisms and cellular responses to genotoxic stress, providing insights into molecular pathways that safeguard genomic stability.

The preferred PNK antibody is PNK Antibody (F-3): sc-271505.