Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PMPCA CRISPR/Cas9 KO Plasmid (h): sc-406691
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PMPCA CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PMPCA genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PMPCA Antibody (B-8): sc-390471
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PMPCA CRISPR/Cas9 KO Plasmid (h) | sc-406691 | 20 µg | $397.00 |
Overview
PMPCA encodes the alpha subunit of mitochondrial processing peptidase (MPP), a heterodimeric metalloprotease that cleaves N-terminal mitochondrial targeting presequences from nuclear-encoded proteins after import into the mitochondrial matrix. Through this maturation step, PMPCA supports assembly and function of oxidative phosphorylation complexes, mitochondrial proteostasis, and broader energy metabolism programs linked to mitochondrial biogenesis. Disruption of PMPCA activity can impair mitochondrial protein maturation, alter respiratory chain performance, and trigger cellular stress responses that intersect with apoptosis and metabolic reprogramming. Variants in PMPCA have been associated with mitochondrial disorders featuring neurodevelopmental and neurodegenerative phenotypes, making it relevant for mechanistic studies of mitochondrial dysfunction.
PMPCA CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PMPCA gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PMPCA together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PMPCA open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PMPCA protein expression.
This CRISPR knockout system enables efficient generation of PMPCA-deficient cell models for investigation of PMPCA signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PMPCA exon(s) critical for PMPCA function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PMPCA genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PMPCA CRISPR/Cas9 KO Plasmid (h) and PMPCA CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PMPCA locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PMPCA HDR Plasmid (h) and PMPCA HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PMPCA homology arms to support homology-directed repair at defined PMPCA target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.