PMCA4b Antibody (JA3): sc-20027
- PMCA4b Antibody (JA3) is a mouse monoclonal IgG1 κ PMCA4b antibody, cited in 7 publications, provided at 200 µg/ml
- raised against purified erythrocyte Ca C2+ pump of human origin with epitope mapping to amino acids 1135-1205
- PMCA4b Antibody (JA3) is recommended for detection of PMCA4b of mouse, rat and human origin by WB, IP, IF and IHC(P)
- Anti-PMCA4b Antibody (JA3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for PMCA4b Antibody (JA3) for WB and IHC(P) applications. This reagent is now offered in a bundle with PMCA4b Antibody (JA3) (see ordering information below).
QUICK LINKS
PMCA4b Antibody (JA3) is a mouse monoclonal IgG1 kappa light chain antibody that detects PMCA4b protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-PMCA4b antibody (JA3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The plasma membrane Ca2+-pumping ATPase (PMCA) family consists of four genes, PMCA1-4, which undergo alternative splicing to produce "b" splice forms that contain PDZ interaction domains, allowing them to interact with different members of the PSD95 family. PMCA4b is predominantly expressed in developing mammary tissue, where PMCA4b plays a crucial role in regulating calcium homeostasis during lactation. The expression of PMCA1b increases while PMCA4b decreases during this period, highlighting the dynamic regulation of calcium transport in response to physiological demands. PMCA4b is cleaved by caspase-3 at Asp 1080, resulting in a fully active fragment that responds more rapidly to calcium fluctuations than its autoinhibited counterpart. Anti-PMCA4b antibody (JA3) is essential for studying PMCA4b′s role in maintaining low cytosolic calcium levels in resting platelets and potential involvement in apoptotic processes, where PMCA4b may help regulate intracellular calcium levels.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PMCA4b Antibody (JA3) References:
- Ca2+-ATPases and their expression in the mammary gland of pregnant and lactating rats. | Reinhardt, TA. and Horst, RL. 1999. Am J Physiol. 276: C796-802. PMID: 10199809
- Ca(2+)-ATPase protein expression in mammary tissue. | Reinhardt, TA., et al. 2000. Am J Physiol Cell Physiol. 279: C1595-602. PMID: 11029307
- Plasma membrane Ca2+-atpase isoforms 2b and 4b interact promiscuously and selectively with members of the membrane-associated guanylate kinase family of PDZ (PSD95/Dlg/ZO-1) domain-containing proteins. | DeMarco, SJ. and Strehler, EE. 2001. J Biol Chem. 276: 21594-600. PMID: 11274188
- Plasma membrane Ca(2+)-ATPase associates with the cytoskeleton in activated platelets through a PDZ-binding domain. | Zabe, M. and Dean, WL. 2001. J Biol Chem. 276: 14704-9. PMID: 11278574
- Plasma membrane Ca2+ATPase isoform 4b is cleaved and activated by caspase-3 during the early phase of apoptosis. | Pászty, K., et al. 2002. J Biol Chem. 277: 6822-9. PMID: 11751908
- Analysis of the tissue-specific distribution of mRNAs encoding the plasma membrane calcium-pumping ATPases and characterization of an alternately spliced form of PMCA4 at the cDNA and genomic levels. | Brandt, P., et al. 1992. J Biol Chem. 267: 4376-85. PMID: 1531651
- Regulation of platelet plasma membrane Ca2+-ATPase by cAMP-dependent and tyrosine phosphorylation. | Dean, WL., et al. 1997. J Biol Chem. 272: 15113-9. PMID: 9182531
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PMCA4b Antibody (JA3) | sc-20027 | 200 µg/ml | $316.00 | |||
PMCA4b Antibody (JA3): m-IgG Fc BP-HRP Bundle | sc-551822 | 200 µg Ab; 10 µg BP | $354.00 | |||
PMCA4b Antibody (JA3) AC | sc-20027 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PMCA4b Antibody (JA3) HRP | sc-20027 HRP | 200 µg/ml | $316.00 | |||
PMCA4b Antibody (JA3) FITC | sc-20027 FITC | 200 µg/ml | $330.00 | |||
PMCA4b Antibody (JA3) PE | sc-20027 PE | 200 µg/ml | $343.00 | |||
PMCA4b Antibody (JA3) Alexa Fluor® 488 | sc-20027 AF488 | 200 µg/ml | $357.00 | |||
PMCA4b Antibody (JA3) Alexa Fluor® 546 | sc-20027 AF546 | 200 µg/ml | $357.00 | |||
PMCA4b Antibody (JA3) Alexa Fluor® 594 | sc-20027 AF594 | 200 µg/ml | $357.00 | |||
PMCA4b Antibody (JA3) Alexa Fluor® 647 | sc-20027 AF647 | 200 µg/ml | $357.00 | |||
PMCA4b Antibody (JA3) Alexa Fluor® 680 | sc-20027 AF680 | 200 µg/ml | $357.00 | |||
PMCA4b Antibody (JA3) Alexa Fluor® 790 | sc-20027 AF790 | 200 µg/ml | $357.00 |