PMCA4b Antibody (2T2): sc-71909

PMCA4b Antibody (2T2) is a mouse monoclonal IgG1 antibody that detects PMCA4b in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). PMCA4b, a member of the plasma membrane Ca2+-pumping ATPase family, plays a crucial role in regulating intracellular calcium levels, which is vital for various cellular processes, including muscle contraction, neurotransmitter release, and cell signaling. PMCA4b is predominantly expressed in developing mammary tissue, where PMCA4b is essential for maintaining calcium homeostasis during lactation. Notably, PMCA4b undergoes post-translational modifications, such as phosphorylation at Tyr 1176 by the src family kinase pp60, which enhances PMCA4b activity and responsiveness to calcium fluctuations. Additionally, during apoptosis, PMCA4b is cleaved by caspase-3, resulting in a fully active fragment that reacts more rapidly to increases in calcium levels compared to its autoinhibited form. PMCA4b maintains low cytosolic calcium levels in resting platelets, underscoring PMCA4b′s importance in cellular function and signaling pathways. Anti-PMCA4b antibody (2T2) serves as an invaluable tool for researchers studying calcium regulation and its implications in various physiological and pathological processes.

The preferred PMCA1/4 antibody is PMCA1/4 Antibody (5F10): sc-20028.