PLEKHA5 CRISPR/Cas9 KO Plasmid (h): sc-408239
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PLEKHA5 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PLEKHA5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PLEKHA5 Antibody (E-2): sc-390311
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PLEKHA5 CRISPR/Cas9 KO Plasmid (h) | sc-408239 | 20 µg | $397.00 |
Overview
PLEKHA5 encodes a pleckstrin homology domain–containing adaptor associated with membrane-proximal signaling and cytoskeletal organization, supporting spatial control of protein complexes in polarized cells. By coupling phosphoinositide-rich membrane domains to regulatory factors, PLEKHA5 has been linked to processes including vesicular trafficking, cell morphology, and context-dependent modulation of proliferative signaling. Altered PLEKHA5 expression or regulation has been reported in cancer genomics and expression studies, consistent with roles in cell-cycle control and migratory phenotypes. These features make PLEKHA5 a useful target for dissecting membrane-dependent signaling nodes that influence growth and cell behavior.
PLEKHA5 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PLEKHA5 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PLEKHA5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PLEKHA5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PLEKHA5 protein expression.
This CRISPR knockout system enables efficient generation of PLEKHA5-deficient cell models for investigation of PLEKHA5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PLEKHA5 exon(s) critical for PLEKHA5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PLEKHA5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PLEKHA5 CRISPR/Cas9 KO Plasmid (h) and PLEKHA5 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PLEKHA5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PLEKHA5 HDR Plasmid (h) and PLEKHA5 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PLEKHA5 homology arms to support homology-directed repair at defined PLEKHA5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.