Anti-PLEKHA5 Antibody (E-2) recommended for detection of PLEKHA5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-PLEKHA5 Antibody (E-2): sc-390311

Anti-PLEKHA5 Antibody (E-2) is rated 4.7 out of 5 by 3.
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Datasheets

    • Anti-PLEKHA5 Antibody (E-2) is a mouse monoclonal IgG2b κ PLEKHA5 antibody, cited in 3 publications, provided at 200 µg/ml
    • raised against amino acids 216-470 mapping within an internal region of PLEKHA5 of human origin
    • Anti-PLEKHA5 Antibody (E-2) is recommended for detection of PLEKHA5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-PLEKHA5 Antibody (E-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PLEKHA5 (E-2): sc-390311.
    • m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PLEKHA5 Antibody (E-2) for WB and IHC(P) applications. These reagents are now offered in bundles with PLEKHA5 Antibody (E-2) (see ordering information below).
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Can PLEKHA5 (E-2): sc-390311 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: AbPolly
Thank you for your question. Yes, PLEKHA5 (E-2): sc-390311 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 4 out of 5 by from good antibody Nice immunoperoxidase staining on formalin fixed, paraffin-embedded human tissue. Dilution used 1:250-1:100
Date published: 2017-02-21
Rated 5 out of 5 by from Produced superb immunoperoxidase cytoplasmic Produced superb immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human kidney tissue. -SCBT QC
Date published: 2015-05-12
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