PLEK2 Antibody (E-11): sc-393831
- PLEK2 Antibody (E-11) is a mouse monoclonal IgG1 κ PLEK2 antibody provided at 200 µg/ml
- raised against amino acids 96-255 mapping within an internal region of PLEK2 of human origin
- PLEK2 Antibody (E-11) is recommended for detection of PLEK2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-PLEK2 Antibody (E-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PLEK2 Antibody (E-11) for WB applications. These reagents are now offered in bundles with PLEK2 Antibody (E-11) (see ordering information below).
QUICK LINKS
SEE ALSO...
PLEK2 Antibody (E-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects PLEK2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PLEK2 (E-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PLEK2, or pleckstrin 2, is a 353 amino acid peripheral membrane protein characterized by the presence of both an N- and a C-terminal pleckstrin homology (PH) domain, along with an intervening DEP domain. PLEK2 plays a crucial role in cytoskeletal dynamics, as PLEK2 is involved in the formation of lamellipodia, which are essential for cell motility and shape changes. PLEK2 is predominantly localized to the cytoskeleton and is expressed in various tissues, with the highest levels found in the thymus, prostate, testis, ovary, small bowel, and large bowel. PLEK2′s interaction with cell membranes facilitates actin rearrangement, which is vital for cellular processes such as migration and adhesion. Notably, while PLEK2 shares high homology with pleckstrin, PLEK2 possesses only a single phosphorylation site and is considered an inefficient substrate for protein kinase C (PKC), which may influence functional dynamics in signaling pathways. The gene encoding PLEK2 is located on human chromosome 14q23.3, highlighting PLEK2′s significance in cellular architecture and function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PLEK2 Antibody (E-11) References:
- Pleckstrin 2, a widely expressed paralog of pleckstrin involved in actin rearrangement. | Hu, MH., et al. 1999. J Biol Chem. 274: 21515-8. PMID: 10419454
- Cloning and expression of pleckstrin 2, a novel member of the pleckstrin family. | Inazu, T., et al. 1999. Biochem Biophys Res Commun. 265: 87-93. PMID: 10548495
- Pleckstrin homology domains and the cytoskeleton. | Lemmon, MA., et al. 2002. FEBS Lett. 513: 71-6. PMID: 11911883
- Cloning, expression and chromosomal assignment of human pleckstrin 2. | Inazu, T., et al. 2005. Mol Biol Rep. 32: 35-40. PMID: 15865208
- PI3K regulates pleckstrin-2 in T-cell cytoskeletal reorganization. | Bach, TL., et al. 2007. Blood. 109: 1147-55. PMID: 17008542
- Pleckstrin-2 selectively interacts with phosphatidylinositol 3-kinase lipid products and regulates actin organization and cell spreading. | Hamaguchi, N., et al. 2007. Biochem Biophys Res Commun. 361: 270-5. PMID: 17658464
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PLEK2 Antibody (E-11) | sc-393831 | 200 µg/ml | $316.00 | |||
PLEK2 Antibody (E-11): m-IgG Fc BP-HRP Bundle | sc-530594 | 200 µg Ab; 10 µg BP | $354.00 | |||
PLEK2 Antibody (E-11): m-IgGκ BP-HRP Bundle | sc-524108 | 200 µg Ab, 40 µg BP | $354.00 | |||
PLEK2 Antibody (E-11): m-IgG1 BP-HRP Bundle | sc-544153 | 200 µg Ab; 20 µg BP | $354.00 | |||
PLEK2 Antibody (E-11) AC | sc-393831 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PLEK2 Antibody (E-11) HRP | sc-393831 HRP | 200 µg/ml | $316.00 | |||
PLEK2 Antibody (E-11) FITC | sc-393831 FITC | 200 µg/ml | $330.00 | |||
PLEK2 Antibody (E-11) PE | sc-393831 PE | 200 µg/ml | $343.00 | |||
PLEK2 Antibody (E-11) Alexa Fluor® 488 | sc-393831 AF488 | 200 µg/ml | $357.00 | |||
PLEK2 Antibody (E-11) Alexa Fluor® 546 | sc-393831 AF546 | 200 µg/ml | $357.00 | |||
PLEK2 Antibody (E-11) Alexa Fluor® 594 | sc-393831 AF594 | 200 µg/ml | $357.00 | |||
PLEK2 Antibody (E-11) Alexa Fluor® 647 | sc-393831 AF647 | 200 µg/ml | $357.00 | |||
PLEK2 Antibody (E-11) Alexa Fluor® 680 | sc-393831 AF680 | 200 µg/ml | $357.00 | |||
PLEK2 Antibody (E-11) Alexa Fluor® 790 | sc-393831 AF790 | 200 µg/ml | $357.00 |