Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PLC δ1 CRISPR/Cas9 KO Plasmid (h): sc-402474
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PLC δ1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PLC δ1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PLC δ1 Antibody (D-7): sc-393464
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PLC δ1 CRISPR/Cas9 KO Plasmid (h) | sc-402474 | 20 µg | $397.00 |
Overview
PLCD1 encodes phospholipase C delta 1 (PLCδ1), a phosphoinositide-specific enzyme that hydrolyzes PIP2 to generate the second messengers IP3 and diacylglycerol, linking membrane lipid signaling to intracellular Ca2+ release and protein kinase C activation. Through these outputs, PLCδ1 contributes to regulation of cytoskeletal dynamics, secretion, membrane trafficking, and stimulus-dependent gene expression across diverse cell types. PLCD1 activity intersects with GPCR- and receptor tyrosine kinase–coupled signaling networks and can modulate downstream MAPK and calcium-dependent transcriptional programs. Altered phosphoinositide/Ca2+ signaling involving PLCD1 has been associated with dysregulated proliferation, differentiation, and stress responses in multiple disease-relevant contexts, supporting its study in cell signaling and mechanistic pathology models.
PLC δ1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PLCD1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PLCD1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PLCD1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PLC δ1 protein expression.
This CRISPR knockout system enables efficient generation of PLCD1-deficient cell models for investigation of PLC δ1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PLCD1 exon(s) critical for PLC δ1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PLCD1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PLC δ1 CRISPR/Cas9 KO Plasmid (h) and PLC δ1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PLCD1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PLC δ1 HDR Plasmid (h) and PLC δ1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PLCD1 homology arms to support homology-directed repair at defined PLCD1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.