PLC β3 Antibody (H-3): sc-133140

PLC β3 Antibody (H-3) is a mouse monoclonal IgG2a antibody that detects phosphoinositide-specific phospholipase C β3 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). PLC β3 plays a pivotal role in cellular signaling by catalyzing the hydrolysis of phosphatidylinositol 4, 5-bisphosphate, leading to the production of inositol 1, 4, 5-triphosphate and diacylglycerol, which are crucial second messengers in numerous physiological processes such as cell growth, differentiation, and apoptosis. Notably, PLC β3 is one of the eight mammalian phospholipase C isozymes and is distinct in its regulatory mechanisms, being activated by G protein subunits and various protein tyrosine kinases that interact with SH2 domains. The presence of these SH2 and SH3 domains in the γ-type enzymes, including PLC β3, allows for intricate regulation and integration of signaling pathways, making PLC β3 essential for understanding receptor-mediated signal transduction. PLC β3′s ability to interact with specific proteins and involvement in critical signaling cascades underscore PLC β3′s importance in both normal physiology and disease states, highlighting anti-PLC β3 antibody (H-3)′s relevance in research and diagnostic applications.

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PLC β3 Antibody (H-3) References:

1. PDZ domain-containing 1 (PDZK1) protein regulates phospholipase C-β3 (PLC-β3)-specific activation of somatostatin by forming a ternary complex with PLC-β3 and somatostatin receptors.  |  Kim, JK., et al. 2012. J Biol Chem. 287: 21012-24. PMID: 22528496
2. Molecular mechanisms of phospholipase C β3 autoinhibition.  |  Lyon, AM., et al. 2014. Structure. 22: 1844-1854. PMID: 25435326
3. Regulation of retinal angiogenesis by phospholipase C-β3 signaling pathway.  |  Ha, JM., et al. 2016. Exp Mol Med. 48: e240. PMID: 27311705
4. Mal protein stabilizes luminal membrane PLC-β3 and negatively regulates ENaC in mouse cortical collecting duct cells.  |  Tuna, KM., et al. 2019. Am J Physiol Renal Physiol. 317: F986-F995. PMID: 31364376
5. The mechanism of Gαq regulation of PLCβ3-catalyzed PIP2 hydrolysis.  |  Falzone, ME. and MacKinnon, R. 2023. Proc Natl Acad Sci U S A. 120: e2315011120. PMID: 37991948
6. FTO-mediated m6A demethylation regulates GnRH expression in the hypothalamus via the PLCβ3/Ca2+/CAMK signalling pathway.  |  Zang, S., et al. 2023. Commun Biol. 6: 1297. PMID: 38129517
7. Phillyrin and its metabolites treat pulmonary embolism by targeting PLCβ3 to inhibit platelet activation.  |  Li, J., et al. 2024. J Ethnopharmacol. 333: 118457. PMID: 38866117
8. Ileal Crohn's Disease Exhibits Reduced Activity of Phospholipase C-β3-Dependent Wnt/β-Catenin Signaling Pathway.  |  Ando, T., et al. 2024. Cells. 13: PMID: 38891118
9. Phospholipase C-β3 is dispensable for vascular constriction but indispensable for vascular hyperplasia.  |  Jin, SY., et al. 2024. Exp Mol Med. 56: 1620-1630. PMID: 38945956
10. Coexpression of ligand-gated P2X and G protein-coupled P2Y receptors in smooth muscle. Preferential activation of P2Y receptors coupled to phospholipase C (PLC)-beta1 via Galphaq/11 and to PLC-beta3 via Gbetagammai3.  |  Murthy, KS. and Makhlouf, GM. 1998. J Biol Chem. 273: 4695-704. PMID: 9468531

The preferred PLC β3 antibody is PLC β3 Antibody (D-7): sc-133231.