Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PLC β3 CRISPR/Cas9 KO Plasmid (h): sc-401181
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PLC β3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PLC β3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PLC β3 Antibody (D-7): sc-133231
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PLC β3 CRISPR/Cas9 KO Plasmid (h) | sc-401181 | 20 µg | $397.00 |
Overview
PLCB3 encodes phospholipase C beta 3 (PLC β3), a membrane-associated effector downstream of GPCR and certain receptor tyrosine kinase signals that hydrolyzes PIP2 to generate the second messengers IP3 and DAG. This reaction drives intracellular Ca2+ mobilization and protein kinase C activation, shaping signal transduction programs that regulate proliferation, migration, secretion, and immune cell function. PLC β3 activity integrates with calcium signaling, MAPK cascades, and cytoskeletal remodeling, linking extracellular cues to transcriptional and metabolic responses. Dysregulated PLCB3-associated signaling has been implicated in oncogenic and inflammatory contexts, making it a useful node for dissecting pathway wiring in disease-relevant cellular models.
PLC β3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PLCB3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PLCB3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PLCB3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PLC β3 protein expression.
This CRISPR knockout system enables efficient generation of PLCB3-deficient cell models for investigation of PLC β3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PLCB3 exon(s) critical for PLC β3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PLCB3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PLC β3 CRISPR/Cas9 KO Plasmid (h) and PLC β3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PLCB3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PLC β3 HDR Plasmid (h) and PLC β3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PLCB3 homology arms to support homology-directed repair at defined PLCB3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.