Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
plakophilin 3 CRISPR/Cas9 KO Plasmid (m): sc-425181
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- plakophilin 3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the plakophilin 3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: plakophilin 3 Antibody (E-10): sc-166655
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
plakophilin 3 CRISPR/Cas9 KO Plasmid (m) | sc-425181 | 20 µg | $397.00 |
Overview
Pkp3 encodes plakophilin 3, an armadillo-repeat protein that localizes to desmosomes and supports cell–cell adhesion by stabilizing desmosomal cadherin–plakoglobin complexes and linking junctions to the keratin intermediate filament network. In mouse tissues, PKP3 contributes to epithelial barrier integrity, tissue architecture, and mechanical resilience under stress. Through its role in desmosome assembly and junctional remodeling, PKP3 intersects with pathways governing cytoskeletal organization, epithelial polarity, and contact-dependent signaling. Altered expression or junctional mislocalization of desmosomal components, including PKP3, is associated with epithelial fragility and has been studied in contexts of inflammation and tumor progression where adhesion and motility programs are dysregulated.
plakophilin 3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Pkp3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Pkp3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Pkp3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish plakophilin 3 protein expression.
This CRISPR knockout system enables efficient generation of Pkp3-deficient cell models for investigation of plakophilin 3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Pkp3 exon(s) critical for plakophilin 3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Pkp3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by plakophilin 3 CRISPR/Cas9 KO Plasmid (m) and plakophilin 3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Pkp3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by plakophilin 3 HDR Plasmid (m) and plakophilin 3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Pkp3 homology arms to support homology-directed repair at defined Pkp3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.