plakophilin 1 CRISPR/Cas9 KO Plasmid (m): sc-422275
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- plakophilin 1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the plakophilin 1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: plakophilin 1 Antibody (10B2): sc-33636
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
plakophilin 1 CRISPR/Cas9 KO Plasmid (m) | sc-422275 | 20 µg | $397.00 |
Overview
Pkp1 encodes plakophilin 1, an armadillo-repeat protein that functions as a core component of desmosomes, linking desmosomal cadherins to intermediate filament networks to support epithelial cell–cell adhesion and tissue integrity. In mouse, plakophilin 1 contributes to desmosome assembly and stabilization, coordinating junctional remodeling with cytoskeletal organization and mechanotransduction. Perturbation of PKP1-dependent adhesion impacts epidermal barrier formation and stress resilience, processes frequently studied in skin and stratified epithelium biology. Because desmosomal dysfunction influences differentiation, wound response, and epithelial homeostasis, Pkp1 is relevant for investigating adhesion-associated pathologies and junctional signaling networks.
plakophilin 1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Pkp1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Pkp1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Pkp1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish plakophilin 1 protein expression.
This CRISPR knockout system enables efficient generation of Pkp1-deficient cell models for investigation of plakophilin 1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Pkp1 exon(s) critical for plakophilin 1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Pkp1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by plakophilin 1 CRISPR/Cas9 KO Plasmid (m) and plakophilin 1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Pkp1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by plakophilin 1 HDR Plasmid (m) and plakophilin 1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Pkp1 homology arms to support homology-directed repair at defined Pkp1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.