plakophilin 1 Antibody (10B2): sc-33636
- plakophilin 1 Antibody (10B2) is a mouse monoclonal IgG1 κ plakophilin 1 antibody, cited in 6 publications, provided at 200 µg/ml
- raised against the N-terminus of plakophilin 1 of human origin, eptiope mapping to amino acids 1-27 of human plakophilin 1
- plakophilin 1 Antibody (10B2) is recommended for detection of plakophilin 1 of mouse, rat and human origin by WB, IP and IF; non cross-reactive with plakophilin 2 or 3
- Anti-plakophilin 1 Antibody (10B2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for plakophilin 1 Antibody (10B2) for WB applications. These reagents are now offered in bundles with plakophilin 1 Antibody (10B2) (see ordering information below).
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plakophilin 1 Antibody (10B2) is a mouse monoclonal IgG1 kappa light chain antibody that detects plakophilin 1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-plakophilin 1 antibody (10B2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Plakophilin 1 plays a crucial role in forming and maintaining desmosomes, which are specialized cell-cell junctions that provide mechanical strength to tissues, particularly in the heart and skin. By linking cadherins to cytoskeletal intermediate filaments, plakophilin 1 is essential for desmosome assembly and stability, thereby influencing cell adhesion and migration. Plakophilin 1′s presence in desmosomes is vital for maintaining tissue integrity under mechanical stress, making plakophilin 1 a key player in the development and function of epithelial tissues. Plakophilin 1 interacts with other plakophilins, such as plakophilin 2, which is critical for heart morphogenesis, and plakophilin 3, which is involved in signaling pathways related to desmosome-dependent adhesion. Plakophilin 1′s ability to regulate desmosomal protein content and assembly underscores plakophilin 1′s importance in cellular architecture and tissue resilience.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
plakophilin 1 Antibody (10B2) References:
- Desmosomal plakophilin 2 as a differentiation marker in normal and malignant tissues. | Mertens, C., et al. 1999. Differentiation. 64: 277-90. PMID: 10374264
- Plakophilin-3, a novel armadillo-like protein present in nuclei and desmosomes of epithelial cells. | Bonné, S., et al. 1999. J Cell Sci. 112 (Pt 14): 2265-76. PMID: 10381383
- The function of plakophilin 1 in desmosome assembly and actin filament organization. | Hatzfeld, M., et al. 2000. J Cell Biol. 149: 209-22. PMID: 10747098
- Plakophilin 1 interferes with plakoglobin binding to desmoplakin, yet together with plakoglobin promotes clustering of desmosomal plaque complexes at cell-cell borders. | Bornslaeger, EA., et al. 2001. J Cell Sci. 114: 727-38. PMID: 11171378
- Nuclear particles containing RNA polymerase III complexes associated with the junctional plaque protein plakophilin 2. | Mertens, C., et al. 2001. Proc Natl Acad Sci U S A. 98: 7795-800. PMID: 11416169
- Protein binding and functional characterization of plakophilin 2. Evidence for its diverse roles in desmosomes and beta -catenin signaling. | Chen, X., et al. 2002. J Biol Chem. 277: 10512-22. PMID: 11790773
- Defining desmosomal plakophilin-3 interactions. | Bonné, S., et al. 2003. J Cell Biol. 161: 403-16. PMID: 12707304
- Lack of plakophilin 1 increases keratinocyte migration and reduces desmosome stability. | South, AP., et al. 2003. J Cell Sci. 116: 3303-14. PMID: 12840072
- Plakophilin 1: an important stabilizer of desmosomes. | South, AP. 2004. Clin Exp Dermatol. 29: 161-7. PMID: 14987275
- Requirement of plakophilin 2 for heart morphogenesis and cardiac junction formation. | Grossmann, KS., et al. 2004. J Cell Biol. 167: 149-60. PMID: 15479741
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
plakophilin 1 Antibody (10B2) | sc-33636 | 200 µg/ml | $322.00 | |||
plakophilin 1 Antibody (10B2): m-IgG Fc BP-HRP Bundle | sc-528478 | 200 µg Ab; 10 µg BP | $361.00 | |||
plakophilin 1 Antibody (10B2): m-IgGκ BP-HRP Bundle | sc-520868 | 200 µg Ab, 40 µg BP | $361.00 | |||
plakophilin 1 Antibody (10B2): m-IgG1 BP-HRP Bundle | sc-542956 | 200 µg Ab; 20 µg BP | $361.00 | |||
plakophilin 1 Antibody (10B2) AC | sc-33636 AC | 500 µg/ml, 25% agarose | $424.00 | |||
plakophilin 1 Antibody (10B2) HRP | sc-33636 HRP | 200 µg/ml | $322.00 | |||
plakophilin 1 Antibody (10B2) FITC | sc-33636 FITC | 200 µg/ml | $336.00 | |||
plakophilin 1 Antibody (10B2) PE | sc-33636 PE | 200 µg/ml | $349.00 | |||
plakophilin 1 Antibody (10B2) Alexa Fluor® 488 | sc-33636 AF488 | 200 µg/ml | $364.00 | |||
plakophilin 1 Antibody (10B2) Alexa Fluor® 546 | sc-33636 AF546 | 200 µg/ml | $364.00 | |||
plakophilin 1 Antibody (10B2) Alexa Fluor® 594 | sc-33636 AF594 | 200 µg/ml | $364.00 | |||
plakophilin 1 Antibody (10B2) Alexa Fluor® 647 | sc-33636 AF647 | 200 µg/ml | $364.00 | |||
plakophilin 1 Antibody (10B2) Alexa Fluor® 680 | sc-33636 AF680 | 200 µg/ml | $364.00 | |||
plakophilin 1 Antibody (10B2) Alexa Fluor® 790 | sc-33636 AF790 | 200 µg/ml | $364.00 |