PKC theta Antibody (E-7): sc-1680

PKC θ Antibody (E-7) is a mouse monoclonal IgG2a kappa light chain antibody designed to target the protein kinase C theta (PRKCQ) protein. PKC θ monoclonal antibody (E-7) recognizes a precise epitope mapping between amino acids 690-707 at the C-terminus of mouse PKC θ, with detection across multiple species, including mouse, rat, and human. PKC θ, also known as protein kinase C θ, is a critical enzyme involved in T-cell activation and the regulation of immune responses, making PKC θ an important target for studies in immunology and therapeutic research. PKC θ encompasses key regulatory roles in cellular processes such as cell growth, differentiation, gene expression, hormone secretion, and membrane function, underscoring PKC θ significance in maintaining cellular homeostasis and signaling pathways. PKC θ monoclonal antibody (E-7) is versatile in applications, effectively performing in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA), which facilitates comprehensive analysis in various experimental settings. Additionally, PKC θ (E-7) antibody is available in both non-conjugated form and multiple conjugated forms, including horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and several Alexa Fluor® conjugates, providing flexibility for diverse research needs. PKC θ research can provide deeper insights into mechanisms underlying immune regulation and identify potential therapeutic targets for autoimmune diseases and cancer.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PKC theta Antibody (E-7) References:

1. Mammalian TOR controls one of two kinase pathways acting upon nPKCdelta and nPKCepsilon.  |  Parekh, D., et al. 1999. J Biol Chem. 274: 34758-64. PMID: 10574945
2. Phosphorylation sites of protein kinase C delta in H2O2-treated cells and its activation by tyrosine kinase in vitro.  |  Konishi, H., et al. 2001. Proc Natl Acad Sci U S A. 98: 6587-92. PMID: 11381116
3. Characterization of TCR-induced phosphorylation of PKCtheta in primary murine lymphocytes.  |  Thebault, S. and Ochoa-Garay, J. 2004. Mol Immunol. 40: 931-42. PMID: 14725789
4. A new member of the protein kinase C family, nPKC theta, predominantly expressed in skeletal muscle.  |  Osada, S., et al. 1992. Mol Cell Biol. 12: 3930-8. PMID: 1508194
5. Calcium-dependent activation of a multifunctional protein kinase by membrane phospholipids.  |  Takai, Y., et al. 1979. J Biol Chem. 254: 3692-5. PMID: 438153
6. Turnover of inositol phospholipids and signal transduction.  |  Nishizuka, Y. 1984. Science. 225: 1365-70. PMID: 6147898
7. The role of protein kinase C in cell surface signal transduction and tumour promotion.  |  Nishizuka, Y. Nature. 308: 693-8. PMID: 6232463
8. Protein kinase C as a possible receptor protein of tumor-promoting phorbol esters.  |  Kikkawa, U., et al. 1983. J Biol Chem. 258: 11442-5. PMID: 6311812
9. Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters.  |  Castagna, M., et al. 1982. J Biol Chem. 257: 7847-51. PMID: 7085651
10. Activation of protein kinase C by tyrosine phosphorylation in response to H2O2.  |  Konishi, H., et al. 1997. Proc Natl Acad Sci U S A. 94: 11233-7. PMID: 9326592

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PKC theta Antibody (E-7) has 24 citations in a variety of scientific publications.

Primary antibodies like Anti-PKC theta Antibody (E-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PKC theta Antibody (E-7) Specifications

Alternate Product Name: PKC theta

Product Clone Name: E-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 690-707

Epitope Target: PKC θ

Epitope Species: mouse

Product Reactivity/Detection: PKC θ

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 24 citations in various scientific publications

Available Conjugates/Forms for PKC theta Antibody (E-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred PKC antibody is PKC Antibody (A-3): sc-17769.