PKC nu Antibody (C-1): sc-376024
- PKC nu Antibody (C-1) is a mouse monoclonal IgG2b κ PKC nu antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 31-69 near the N-terminus of PKC ν of human origin
- PKC nu Antibody (C-1) is recommended for detection of PKC ν of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including bovine
- Anti-PKC nu Antibody (C-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP is the preferred secondary detection reagent for PKC nu Antibody (C-1) for WB and IHC(P) applications. This reagent is now offered in a bundle with PKC nu Antibody (C-1) (see ordering information below).
PKC ν (C-1) is a mouse monoclonal IgG2b kappa light chain antibody that detects PKC nu protein of mouse, rat and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP) and enzyme-linked immunosorbent assay (ELISA). Anti-PKC nu antibody (C-1) is available as both non-conjugated form, as well as multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC) and multiple Alexa Fluor® conjugates. Members of the protein kinase C (PKC) family play a key regulatory role in cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity depended on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes, including conventional (c) PKC isoforms (α, βI, βII and γ) and novel (n) PKC isoforms (δ, ε, ζ, η, θ, λ/ι, µ and ν). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in cofactor dependencies. For instance, PKC δ and ε kinase activities are independent of Ca2+, while most other PKC members possess phorbol ester-binding activities and kinase activities.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PKC nu Antibody (C-1) References:
- A new member of the protein kinase C family, nPKC theta, predominantly expressed in skeletal muscle. | Osada, S., et al. 1992. Mol Cell Biol. 12: 3930-8. PMID: 1508194
- Structural and functional diversities of a family of signal transducing protein kinases, protein kinase C family; two distinct classes of PKC, conventional cPKC and novel nPKC. | Ohno, S., et al. 1991. Adv Enzyme Regul. 31: 287-303. PMID: 1877391
- Expression and characterization of protein kinase C-delta. | Olivier, AR. and Parker, PJ. 1991. Eur J Biochem. 200: 805-10. PMID: 1915352
- Calcium-dependent activation of a multifunctional protein kinase by membrane phospholipids. | Takai, Y., et al. 1979. J Biol Chem. 254: 3692-5. PMID: 438153
- Turnover of inositol phospholipids and signal transduction. | Nishizuka, Y. 1984. Science. 225: 1365-70. PMID: 6147898
- The role of protein kinase C in cell surface signal transduction and tumour promotion. | Nishizuka, Y. Nature. 308: 693-8. PMID: 6232463
- Protein kinase C as a possible receptor protein of tumor-promoting phorbol esters. | Kikkawa, U., et al. 1983. J Biol Chem. 258: 11442-5. PMID: 6311812
- Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters. | Castagna, M., et al. 1982. J Biol Chem. 257: 7847-51. PMID: 7085651
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PKC nu Antibody (C-1) | sc-376024 | 200 µg/ml | $322.00 | |||
PKC nu Antibody (C-1): m-IgG2b BP-HRP Bundle | sc-548750 | 200 µg Ab; 10 µg BP | $361.00 | |||
PKC nu Antibody (C-1) AC | sc-376024 AC | 500 µg/ml, 25% agarose | $424.00 | |||
PKC nu Antibody (C-1) HRP | sc-376024 HRP | 200 µg/ml | $322.00 | |||
PKC nu Antibody (C-1) FITC | sc-376024 FITC | 200 µg/ml | $336.00 | |||
PKC nu Antibody (C-1) PE | sc-376024 PE | 200 µg/ml | $349.00 | |||
PKC nu Antibody (C-1) Alexa Fluor® 488 | sc-376024 AF488 | 200 µg/ml | $364.00 | |||
PKC nu Antibody (C-1) Alexa Fluor® 546 | sc-376024 AF546 | 200 µg/ml | $364.00 | |||
PKC nu Antibody (C-1) Alexa Fluor® 594 | sc-376024 AF594 | 200 µg/ml | $364.00 | |||
PKC nu Antibody (C-1) Alexa Fluor® 647 | sc-376024 AF647 | 200 µg/ml | $364.00 | |||
PKC nu Antibody (C-1) Alexa Fluor® 680 | sc-376024 AF680 | 200 µg/ml | $364.00 | |||
PKC nu Antibody (C-1) Alexa Fluor® 790 | sc-376024 AF790 | 200 µg/ml | $364.00 | |||
PKC nu (C-1) Neutralizing Peptide | sc-376024 P | 100 µg/0.5 ml | $69.00 |