PKC beta II Antibody (F-7) recommended for detection of cPKC βII of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA

PKC βII Antibody (F-7): sc-13149

PKC βII Antibody (F-7) is rated 4.9 out of 5 by 18.
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Datasheets

    • PKC βII Antibody (F-7) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 657-673 at the C-terminus of PKC βII of human origin
    • Anti-PKC beta II Antibody (F-7) is recommended for detection of cPKC βII of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA; also reactive with additional species, including and bovine and porcine
    • Anti-PKC beta II Antibody (F-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-13149 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PKC βII (F-7): sc-13149.

I have some monkey samples I would like to use for WB, do you know if this antibody will cross-react?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Service 11
Date published: 2017-01-12

Do you have any tips for performing a double stain with only mouse monoclonal antibodies?

Asked by: Cweed
We recommend using directly conjugated primary antibodies. This product, sc-13149, is available conjugated to FITC, AF488, and AF647.
Answered by: Technical Service 11
Date published: 2017-01-12
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Rated 5 out of 5 by from Worth investing Helped our Team's research in the experiments planned. Arrived on time and in a proper condition.
Date published: 2017-10-04
Rated 5 out of 5 by from good performance This PKC βII antibody very good performance. Without any need to optimiezed the protocol. It is specific and sensitive to the target protein at 80kda by 1:500.
Date published: 2017-09-02
Rated 5 out of 5 by from very useful antibody This anibody is cost effectiveness than other supplier’s antibody ,the result shows specific binding, high conceration at 1:1000.
Date published: 2017-07-08
Rated 5 out of 5 by from great antibody for western blotting We used this antibody to examine its expression in postsynaptic density fractions purified from neonatal mouse brain by Western blotting. It worked very well!
Date published: 2017-05-24
Rated 5 out of 5 by from Good results Good results by WB for human PBMC, showed single band with exact molecular weight.
Date published: 2017-05-20
Rated 5 out of 5 by from High effency Single strong band at correct mw in human liver tissue, very good antibody.
Date published: 2017-04-29
Rated 5 out of 5 by from Very efficient PKC βII antibody works well. We use dilution 1:500 got specific band,strong wb with low background, also good for staining.
Date published: 2017-04-16
Rated 5 out of 5 by from Antibody is really great Antibody provides clear and specific results,antibody is really great.
Date published: 2017-02-06
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