PKC beta I Antibody (E-3) recommended for detection of PKC βI of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA

PKC βI Antibody (E-3): sc-8049

PKC βI Antibody (E-3) is rated 4.6 out of 5 by 14.
  • y_2020, m_7, d_2, h_15
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.9
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_14
  • loc_en_US, sid_8049, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 142ms
  • REVIEWS, PRODUCT
5
10
4
2
3
2
2
0
1
0

Datasheets

    • Anti-PKC βI Antibody (E-3) is a mouse monoclonal IgG1 (kappa light chain) PKC βI antibody provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 656-671 at the C-terminus of PKC βI of human origin
    • PKC beta I Antibody (E-3) is recommended for detection of PKC βI of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and canine, bovine, porcine and avian
    • Anti-PKC beta I Antibody (E-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-8049 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PKC βI (E-3): sc-8049.

Can the blocking peptide be used as a positive control in Western Blot?

Asked by: AbPolly
Thank you for your question. The blocking peptide, sc-8049 P, is a short synthetic peptide and is intended for use as a negative control. It is not appropriate for use as a positive control in Western Blot.
Answered by: Technical Support 9
Date published: 2017-01-24
  • y_2020, m_7, d_2, h_15CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.9
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_en_US, sid_8049, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 137ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Good I tested this antibody in Jan 2018. The bands are clear and the pattern detected matches the data sheet.
Date published: 2018-02-13
Rated 5 out of 5 by from It's perfect We get specific signal for the protein at the right molecular weight when we use PKC βI Antibody of the cell. It's the very efficient antibody indeed.
Date published: 2017-10-21
Rated 5 out of 5 by from The antibody is really great PKC βI Antibody is highly suitable to show cellular staining. Show us the strong signal with low background and the baseband is perfect.
Date published: 2017-10-18
Rated 4 out of 5 by from good antibody Used the antibody at the WB,it is very useful for my experiment! And we will buy some.
Date published: 2017-10-11
Rated 3 out of 5 by from slightly worked Overexpressed HA-PKCbI in 293 cells was weakly detected. HA-PKCa was cross reacted. endogenous PKCbI was weakly detected in 293 cell lysates. 1/1000 dil in 2% BSA TBST, o/n reaction.
Date published: 2017-07-05
Rated 5 out of 5 by from helpful for my experiment Used the antibody at the WB,it is very useful for my experiment!
Date published: 2017-06-21
Rated 3 out of 5 by from Good results I tested this antibody by western blot by using 0.2ug/ml in human peripheral blood mononuclear cell (PBMC). good for WB
Date published: 2017-05-22
Rated 5 out of 5 by from great antibody for flow cytometry This antibody worked very well for flow cytometry using SupT1 cells
Date published: 2017-03-07
  • y_2020, m_7, d_2, h_15
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.9
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_14
  • loc_en_US, sid_8049, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 10ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2020 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.