PKC beta 1 Antibody (E-3): sc-8049
- PKC beta 1 Antibody (E-3) is a mouse monoclonal IgG1 κ PKC beta 1 antibody, cited in 51 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 656-671 at the C-terminus of PKC βI of human origin
- PKC beta 1 Antibody (E-3) is recommended for detection of PKC βI of mouse, rat, human and origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and canine, bovine, porcine and avian
- Anti-PKC beta 1 Antibody (E-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PKC beta 1 Antibody (E-3) for WB and IHC(P) applications. These reagents are now offered in bundles with PKC beta 1 Antibody (E-3) (see ordering information below).
PKC βI Antibody (E-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects PKC beta I protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PKC βI (E-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PKC beta I protein, also known as PRKCB, is a crucial member of the protein kinase C (PKC) family, which plays a significant role in regulating diverse cellular functions such as cell growth, differentiation, gene expression, and hormone secretion. PKC beta I is primarily located in the cytoplasm and translocates to the membrane upon activation, which is essential for its role in signal transduction pathways. This translocation allows PKC beta I to interact with various substrates and participate in critical cellular processes, including the modulation of cell survival and apoptosis. Activation of PKC beta I is influenced by diacylglycerols and phorbol esters, which bind to PKC beta I and promote kinase activity. Understanding PKC beta I localization and function is important for elucidating involvement in various diseases, including cancer and cardiovascular disorders, making PKC βI (E-3) monoclonal antibody an invaluable tool for researchers studying these pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PKC beta 1 Antibody (E-3) References:
- A new member of the protein kinase C family, nPKC theta, predominantly expressed in skeletal muscle. | Osada, S., et al. 1992. Mol Cell Biol. 12: 3930-8. PMID: 1508194
- Structural and functional diversities of a family of signal transducing protein kinases, protein kinase C family; two distinct classes of PKC, conventional cPKC and novel nPKC. | Ohno, S., et al. 1991. Adv Enzyme Regul. 31: 287-303. PMID: 1877391
- Expression and characterization of protein kinase C-delta. | Olivier, AR. and Parker, PJ. 1991. Eur J Biochem. 200: 805-10. PMID: 1915352
- Calcium-dependent activation of a multifunctional protein kinase by membrane phospholipids. | Takai, Y., et al. 1979. J Biol Chem. 254: 3692-5. PMID: 438153
- Turnover of inositol phospholipids and signal transduction. | Nishizuka, Y. 1984. Science. 225: 1365-70. PMID: 6147898
- The role of protein kinase C in cell surface signal transduction and tumour promotion. | Nishizuka, Y. Nature. 308: 693-8. PMID: 6232463
- Protein kinase C as a possible receptor protein of tumor-promoting phorbol esters. | Kikkawa, U., et al. 1983. J Biol Chem. 258: 11442-5. PMID: 6311812
- Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters. | Castagna, M., et al. 1982. J Biol Chem. 257: 7847-51. PMID: 7085651
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PKC beta 1 Antibody (E-3) | sc-8049 | 200 µg/ml | $316.00 | |||
PKC beta 1 Antibody (E-3): m-IgG Fc BP-HRP Bundle | sc-528214 | 200 µg Ab; 10 µg BP | $354.00 | |||
PKC beta 1 Antibody (E-3): m-IgGκ BP-HRP Bundle | sc-520537 | 200 µg Ab, 40 µg BP | $354.00 | |||
PKC beta 1 Antibody (E-3) AC | sc-8049 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PKC beta 1 Antibody (E-3) HRP | sc-8049 HRP | 200 µg/ml | $316.00 | |||
PKC beta 1 Antibody (E-3) FITC | sc-8049 FITC | 200 µg/ml | $330.00 | |||
PKC beta 1 Antibody (E-3) PE | sc-8049 PE | 200 µg/ml | $343.00 | |||
PKC beta 1 Antibody (E-3) Alexa Fluor® 488 | sc-8049 AF488 | 200 µg/ml | $357.00 | |||
PKC beta 1 Antibody (E-3) Alexa Fluor® 546 | sc-8049 AF546 | 200 µg/ml | $357.00 | |||
PKC beta 1 Antibody (E-3) Alexa Fluor® 594 | sc-8049 AF594 | 200 µg/ml | $357.00 | |||
PKC beta 1 Antibody (E-3) Alexa Fluor® 647 | sc-8049 AF647 | 200 µg/ml | $357.00 | |||
PKC beta 1 Antibody (E-3) Alexa Fluor® 680 | sc-8049 AF680 | 200 µg/ml | $357.00 | |||
PKC beta 1 Antibody (E-3) Alexa Fluor® 790 | sc-8049 AF790 | 200 µg/ml | $357.00 | |||
PKC beta 1 (E-3) Neutralizing Peptide | sc-8049 P | 100 µg/0.5 ml | $68.00 |