PKAγ cat Antibody (A-4): sc-514087
- PKAγ cat Antibody (A-4) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 322-350 at the C-terminus of PKAγ cat of human origin
- Anti-PKA gamma cat Antibody (A-4) is recommended for detection of PKAγ catalytic subunit of mouse, rat, human and mink origin by WB, IP, IF, IHC(P) and ELISA
- Anti-PKA gamma cat Antibody (A-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PKAγ cat Antibody (A-4) for WB and IHC(P) applications. These reagents are now offered in bundles with PKAγ cat Antibody (A-4) (see ordering information below).
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PKAγ cat Antibody (A-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects PKAγ catalytic subunit of mouse, rat, human, and mink origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). PKAγ cat (A-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PKAγ plays a crucial role in cellular signaling pathways as a key component of the cAMP-dependent protein kinase (PKA) complex, which is activated by the second messenger cyclic AMP (cAMP). Upon binding of cAMP to the regulatory subunits of the PKA holoenzyme, active catalytic subunits are released, leading to phosphorylation of various target proteins, including transcription factors. PKAγ cat participates in phosphorylating cAMP response element binding protein (CREB) at Serine 133, essential for transcriptional activation of genes responsive to cAMP signaling. This phosphorylation event facilitates recruitment of transcriptional machinery, linking PKA activity to gene expression regulation, vital for processes such as cell proliferation, metabolism, and differentiation. Distinct structural features of PKAγ, including unique amino acid sequence and regulatory mechanisms, underscore PKAγ′s importance in mediating diverse physiological responses to extracellular signals.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PKAγ cat Antibody (A-4) References:
- Molecular cloning of a tissue-specific protein kinase (C gamma) from human testis--representing a third isoform for the catalytic subunit of cAMP-dependent protein kinase. | Beebe, SJ., et al. 1990. Mol Endocrinol. 4: 465-75. PMID: 2342480
- Cyclic AMP stimulates somatostatin gene transcription by phosphorylation of CREB at serine 133. | Gonzalez, GA. and Montminy, MR. 1989. Cell. 59: 675-80. PMID: 2573431
- A cDNA clone encoding human cAMP-dependent protein kinase catalytic subunit C alpha. | Maldonado, F. and Hanks, SK. 1988. Nucleic Acids Res. 16: 8189-90. PMID: 2843813
- Phosphorylation-dephosphorylation of enzymes. | Krebs, EG. and Beavo, JA. 1979. Annu Rev Biochem. 48: 923-59. PMID: 38740
- Activation of protein kinase by physiological concentrations of cyclic AMP. | Beavo, JA., et al. 1974. Proc Natl Acad Sci U S A. 71: 3580-3. PMID: 4372627
- Signal transduction through the cAMP-dependent protein kinase. | Meinkoth, JL., et al. 1993. Mol Cell Biochem. 127-128: 179-86. PMID: 7935349
- Transcription factors. CREB takes CBP to tango. | Nordheim, A. 1994. Nature. 370: 177-8. PMID: 8028657
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PKAγ cat Antibody (A-4) | sc-514087 | 200 µg/ml | $316.00 | |||
PKAγ cat Antibody (A-4): m-IgG Fc BP-HRP Bundle | sc-531026 | 200 µg Ab; 10 µg BP | $354.00 | |||
PKAγ cat Antibody (A-4): m-IgGκ BP-HRP Bundle | sc-524601 | 200 µg Ab, 40 µg BP | $354.00 | |||
PKAγ cat Antibody (A-4) AC | sc-514087 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PKAγ cat Antibody (A-4) HRP | sc-514087 HRP | 200 µg/ml | $316.00 | |||
PKAγ cat Antibody (A-4) FITC | sc-514087 FITC | 200 µg/ml | $330.00 | |||
PKAγ cat Antibody (A-4) PE | sc-514087 PE | 200 µg/ml | $343.00 | |||
PKAγ cat Antibody (A-4) Alexa Fluor® 488 | sc-514087 AF488 | 200 µg/ml | $357.00 | |||
PKAγ cat Antibody (A-4) Alexa Fluor® 546 | sc-514087 AF546 | 200 µg/ml | $357.00 | |||
PKAγ cat Antibody (A-4) Alexa Fluor® 594 | sc-514087 AF594 | 200 µg/ml | $357.00 | |||
PKAγ cat Antibody (A-4) Alexa Fluor® 647 | sc-514087 AF647 | 200 µg/ml | $357.00 | |||
PKAγ cat Antibody (A-4) Alexa Fluor® 680 | sc-514087 AF680 | 200 µg/ml | $357.00 | |||
PKAγ cat Antibody (A-4) Alexa Fluor® 790 | sc-514087 AF790 | 200 µg/ml | $357.00 | |||
PKAγ cat (A-4) Neutralizing Peptide | sc-514087 P | 100 µg/0.5 ml | $68.00 |