PKAα cat Antibody (B-5): sc-28316
- PKAα cat Antibody (B-5) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 322-351 at the C-terminus of PKAα cat of human origin
- recommended for detection of PKAα catalytic subunit of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, bovine and porcine; may cross-react with β and γ subunits
- See PKAα cat (A-2): sc-28315 for PKAα cat antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PKAα cat Antibody (B-5) for WB and IHC(P) applications. These reagents are now offered in bundles with PKAα cat Antibody (B-5) (see ordering information below).
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PKAα cat Antibody (B-5) is a mouse monoclonal IgG1 kappa light chain antibody designed to target the protein kinase A (PKA) alpha catalytic subunit (PRKACA) with exceptional accuracy. Anti-PKAα cat antibody (B-5) recognizes an epitope mapped to amino acids 322-351 at the C-terminus of the human PKAα catalytic subunit, ensuring reliable detection in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA) applications across multiple species, including mouse, rat, and human, as well as additional species such as equine, bovine, and porcine, though may exhibit cross-reactivity with the β and γ subunits of PKA. PKAα cat is a critical component of the cAMP-dependent protein kinase A (PKA) signaling pathway, which plays a pivotal role in regulating cellular processes including cell proliferation, ion transport, metabolism, and gene transcription. Located primarily in the cytoplasm under resting conditions, PKAα cat translocates to the nucleus upon activation by cyclic AMP (cAMP), where PKAα cat phosphorylates key transcription factors such as CREB (cAMP response element-binding protein) on serine 133. This phosphorylation is essential for the initiation of gene transcription, linking extracellular signals to the regulation of gene expression and influencing cellular function and behavior. The precise localization and activation of PKAα cat within the cell are crucial for appropriate cellular responses to various stimuli, making PKAα cat a vital target for research into signal transduction pathways and their implications in numerous diseases. PKAα cat monoclonal antibody (B-5) is available in both non-conjugated form and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, providing versatile options to accommodate diverse experimental requirements and facilitating advanced studies into the mechanisms of PKA-mediated cellular processes and their spatial dynamics within the cell.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PKAα cat Antibody (B-5) | sc-28316 | 200 µg/ml | $322.00 | |||
PKAα cat Antibody (B-5): m-IgG Fc BP-HRP Bundle | sc-536711 | 200 µg Ab; 10 µg BP | $361.00 | |||
PKAα cat Antibody (B-5): m-IgGκ BP-HRP Bundle | sc-533871 | 200 µg Ab; 40 µg BP | $361.00 | |||
PKAα cat Antibody (B-5): m-IgG1 BP-HRP Bundle | sc-544777 | 200 µg Ab; 20 µg BP | $361.00 | |||
PKAα cat (B-5) Neutralizing Peptide | sc-28316 P | 100 µg/0.5 ml | $69.00 |