PKAα/β/γ cat Antibody (G-6): sc-390548

PKAα/β/γ cat Antibody (G-6) is a mouse monoclonal IgG1 kappa light chain antibody designed to detect the catalytic subunits alpha, beta, and gamma of protein kinase A (PKA) across multiple species, including mouse, rat, human, equine, canine, bovine, and porcine, through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PKAα/β/γ cat monoclonal antibody (G-6) is raised against a precise epitope encompassing amino acids 231-286 near the C-terminus of the human PKAα catalytic subunit, ensuring selective and accurate recognition of PKAα, PKAβ, and PKAγ isoforms. Protein kinase A is a crucial cAMP-dependent enzyme that plays a vital role in regulating diverse cellular functions, including cell proliferation, ion transport, metabolism, and gene transcription. Structurally, PKA exists as a tetrameric holoenzyme composed of two regulatory and two catalytic subunits. Upon binding of the second messenger cyclic AMP (cAMP) to the regulatory subunits, the catalytic subunits are released and translocate to specific locations within the cell, such as the cytoplasm and nucleus. This translocation is essential for PKA′s function, as PKA catalytic subunits phosphorylate target proteins in different cellular compartments, thereby modulating various signaling pathways. In the nucleus, active PKA phosphorylates the cAMP response element-binding protein (CREB) on Serine 133, facilitating the recruitment of transcription factors like TFIIB and TBP1 to initiate gene transcription. The precise localization of PKA catalytic subunits is critical for the spatial and temporal regulation of these signaling events, ensuring that cellular responses are appropriately coordinated and executed. Anti-PKAα/β/γ cat antibody (G-6) enables researchers to effectively study the dynamic localization and functional interactions of PKA within cells, providing valuable insights into PKA′s role in both normal physiological processes and various pathological conditions.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PKAα/β/γ cat Antibody (G-6) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-PKAα/β/γ cat Antibody (G-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PKAα/β/γ cat Antibody (G-6) Specifications

Alternate Product Name: PRKACA, PRKACB, PRKACG

Product Clone Name: G-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 231-286

Epitope Target: PKAα catalytic subunit

Epitope Species: human

Product Reactivity/Detection: PKAα/β cat of mouse, rat and human origin, and PKAγ cat of human origin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for PKAα/β/γ cat Antibody (G-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred PKAα cat antibody is PKAα cat Antibody (A-2): sc-28315.