PKA IIα reg Antibody (H-12): sc-137220

PKA IIα reg Antibody (H-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects PKA II alpha reg protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PKA IIα reg (H-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PKA II alpha reg protein, also called protein kinase cAMP-dependent type II regulatory subunit alpha, PRKAR2A, PKR2, or RIIα, plays a crucial role in cellular signaling pathways by acting as a regulatory subunit of the protein kinase A (PKA) complex, which is activated by the second messenger cyclic AMP (cAMP). Upon binding of cAMP to regulatory subunits, active catalytic subunits are released, leading to phosphorylation of various target proteins, including transcription factors. This phosphorylation is essential for mediating diverse cellular responses such as gene transcription, metabolism regulation, and cell proliferation. PKA II alpha reg interacts with specific proteins, including the cAMP response element binding protein (CREB), which is phosphorylated at serine 133, facilitating recruitment of transcriptional machinery to initiate gene expression. PKA′s ability to translocate to the nucleus and influence transcription underscores PKA II alpha reg′s importance in cellular responses to external signals, making anti-PKA IIα reg antibody (H-12) a valuable tool for studying these critical signaling pathways in human cells.

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PKA IIα reg Antibody (H-12) References:

1. Cyclic AMP stimulates somatostatin gene transcription by phosphorylation of CREB at serine 133.  |  Gonzalez, GA. and Montminy, MR. 1989. Cell. 59: 675-80. PMID: 2573431
2. Association between polymorphisms of prokineticin receptor (PKR1 rs4627609 and PKR2 rs6053283) and recurrent pregnancy loss.  |  Cao, YL., et al. 2016. J Zhejiang Univ Sci B. 17: 218-24. PMID: 26984842
3. PKR2 and β-catenin genes regulates pancreatic cancer chemosensitivity.  |  Zhong, RL., et al. 2017. Eur Rev Med Pharmacol Sci. 21: 48-54. PMID: 28121357
4. A cDNA clone encoding human cAMP-dependent protein kinase catalytic subunit C alpha.  |  Maldonado, F. and Hanks, SK. 1988. Nucleic Acids Res. 16: 8189-90. PMID: 2843813
5. Role of N-Linked Glycosylation in PKR2 Trafficking and Signaling.  |  Verdinez, JA. and Sebag, JA. 2021. Front Neurosci. 15: 730417. PMID: 34483834
6. PRKAR2A-derived circular RNAs promote the malignant transformation of colitis and distinguish patients with colitis-associated colorectal cancer.  |  Wan, D., et al. 2022. Clin Transl Med. 12: e683. PMID: 35184406
7. Organoid models of fibrolamellar carcinoma mutations reveal hepatocyte transdifferentiation through cooperative BAP1 and PRKAR2A loss.  |  Rüland, L., et al. 2023. Nat Commun. 14: 2377. PMID: 37137901
8. Phosphorylation-dephosphorylation of enzymes.  |  Krebs, EG. and Beavo, JA. 1979. Annu Rev Biochem. 48: 923-59. PMID: 38740
9. Activation of protein kinase by physiological concentrations of cyclic AMP.  |  Beavo, JA., et al. 1974. Proc Natl Acad Sci U S A. 71: 3580-3. PMID: 4372627
10. Signal transduction through the cAMP-dependent protein kinase.  |  Meinkoth, JL., et al. 1993. Mol Cell Biochem. 127-128: 179-86. PMID: 7935349
11. Transcription factors. CREB takes CBP to tango.  |  Nordheim, A. 1994. Nature. 370: 177-8. PMID: 8028657

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PKA IIα reg Antibody (H-12) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-PKA IIα reg Antibody (H-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PKA IIα reg Antibody (H-12) Specifications

Alternate Product Name: PRKAR2A

Product Clone Name: H-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 21-100 deletion 39-54

Epitope Target: PKA IIα reg

Epitope Species: human

Product Reactivity/Detection: PKA IIα reg

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for PKA IIα reg Antibody (H-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred p-PKA IIα reg antibody is p-PKA IIα reg Antibody (C-5): sc-377575.