Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PKAγ cat Antibody (A-4): sc-514087. Western blot analysis of PKAγ cat expression in PC-3 (A) and Hs 181 Tes (C) whole cell lysates and human testis tissue extract (B).
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PKAγ cat CRISPR/Cas9 KO Plasmid (h): sc-400874
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PKAγ cat CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PKAγ cat genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PKAγ cat Antibody (A-4): sc-514087
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PKAγ cat CRISPR/Cas9 KO Plasmid (h) | sc-400874 | 20 µg | $397.00 |