PJA2 Antibody (H-4): sc-390137
- PJA2 Antibody (H-4) is a mouse monoclonal IgG2b κ PJA2 antibody, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 47-83 near the N-terminus of PJA2 of human origin
- PJA2 Antibody (H-4) is recommended for detection of PJA2 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-PJA2 Antibody (H-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PJA2 Antibody (H-4) for WB applications. These reagents are now offered in bundles with PJA2 Antibody (H-4) (see ordering information below).
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PJA2 Antibody (H-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects PJA2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PJA2 (H-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PJA2, also known as E3 ubiquitin-protein ligase praja-2, RNF131, or Neurodap1, is a 708 amino acid protein characterized by the presence of a ring-type zinc finger and exists in two alternatively spliced isoforms. PJA2 plays a crucial role in the ubiquitin-proteasome system, which is essential for regulating protein degradation and maintaining cellular homeostasis. PJA2 is significantly conserved across various species, including chimpanzees, dogs, cows, mice, rats, chickens, and zebrafish, and shares 52% identity with PJA1, a protein implicated in protein ubiquitination in the brain and associated with X-linked mental retardation. Encoded by a gene located on human chromosome 5q21.3, PJA2 is primarily localized to the membranes of the endoplasmic reticulum and Golgi apparatus, where PJA2 participates in E2-dependent, E3 ubiquitin-protein ligase activity. PJA2 interacts with a variety of E2 ubiquitin-conjugating enzymes, such as UBE2D2, in vitro, highlighting PJA2′s importance in cellular signaling pathways and protein turnover.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PJA2 Antibody (H-4) References:
- A RING finger protein Praja1 regulates Dlx5-dependent transcription through its ubiquitin ligase activity for the Dlx/Msx-interacting MAGE/Necdin family protein, Dlxin-1. | Sasaki, A., et al. 2002. J Biol Chem. 277: 22541-6. PMID: 11959851
- PJA1, encoding a RING-H2 finger ubiquitin ligase, is a novel human X chromosome gene abundantly expressed in brain. | Yu, P., et al. 2002. Genomics. 79: 869-74. PMID: 12036302
- TP53 mutations are associated with a particular pattern of genomic imbalances in breast carcinomas. | Kleivi, K., et al. 2005. J Pathol. 207: 14-9. PMID: 16007576
- Gene expression profiling of rat cerebral cortex development using cDNA microarrays. | Lee, KH., et al. 2009. Neurochem Res. 34: 1030-8. PMID: 18987971
- Characterization of a de novo balanced translocation in a patient with moderate mental retardation and dysmorphic features. | Haddad, MR., et al. 2009. Eur J Med Genet. 52: 211-7. PMID: 19379847
- KSR2 is a calcineurin substrate that promotes ERK cascade activation in response to calcium signals. | Dougherty, MK., et al. 2009. Mol Cell. 34: 652-62. PMID: 19560418
- Praja1, a novel gene encoding a RING-H2 motif in mouse development. | Mishra, L., et al. 1997. Oncogene. 15: 2361-8. PMID: 9393880
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PJA2 Antibody (H-4) | sc-390137 | 200 µg/ml | $322.00 | |||
PJA2 Antibody (H-4): m-IgG Fc BP-HRP Bundle | sc-530076 | 200 µg Ab; 10 µg BP | $361.00 | |||
PJA2 Antibody (H-4): m-IgGκ BP-HRP Bundle | sc-523421 | 200 µg Ab, 40 µg BP | $361.00 | |||
PJA2 Antibody (H-4) AC | sc-390137 AC | 500 µg/ml, 25% agarose | $424.00 | |||
PJA2 Antibody (H-4) HRP | sc-390137 HRP | 200 µg/ml | $322.00 | |||
PJA2 Antibody (H-4) FITC | sc-390137 FITC | 200 µg/ml | $336.00 | |||
PJA2 Antibody (H-4) PE | sc-390137 PE | 200 µg/ml | $349.00 | |||
PJA2 Antibody (H-4) Alexa Fluor® 488 | sc-390137 AF488 | 200 µg/ml | $364.00 | |||
PJA2 Antibody (H-4) Alexa Fluor® 546 | sc-390137 AF546 | 200 µg/ml | $364.00 | |||
PJA2 Antibody (H-4) Alexa Fluor® 594 | sc-390137 AF594 | 200 µg/ml | $364.00 | |||
PJA2 Antibody (H-4) Alexa Fluor® 647 | sc-390137 AF647 | 200 µg/ml | $364.00 | |||
PJA2 Antibody (H-4) Alexa Fluor® 680 | sc-390137 AF680 | 200 µg/ml | $364.00 | |||
PJA2 Antibody (H-4) Alexa Fluor® 790 | sc-390137 AF790 | 200 µg/ml | $364.00 | |||
PJA2 (H-4) Neutralizing Peptide | sc-390137 P | 100 µg/0.5 ml | $69.00 |