Pitx1 Antibody (G-4): sc-271435

Pitx1 Antibody (G-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects Pitx1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Pitx1 antibody (G-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Pitx1 plays a crucial role in limb development, particularly in the hindlimb, where Pitx1 is involved in the regulation of morphogenesis and growth patterns. Pitx1 expression is notably observed in the developing anterior pituitary gland and the first branchial arch, highlighting Pitx1′s importance in various developmental processes. Deletion of Pitx1 locus has been shown to lead to a significant reduction in the expression of the hindlimb-specific marker T-box factor (TBX4), indicating Pitx1′s essential role in proper development of hindlimb structures. Pitx1 interacts with other transcription factors, including Pitx2, which regulates cardiac positioning and tooth morphogenesis, thereby underscoring the interconnected roles of these proteins in developmental biology. Genes encoding Pitx1 and Pitx2 are located on human chromosomes 5q31.1 and 4q25, respectively, and their functional interplay is vital for maintaining morphological distinctions between forelimbs and hindlimbs.

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Pitx1 Antibody (G-4) References:

1. Role of the Bicoid-related homeodomain factor Pitx1 in specifying hindlimb morphogenesis and pituitary development.  |  Szeto, DP., et al. 1999. Genes Dev. 13: 484-94. PMID: 10049363
2. A model for the development of the hypothalamic-pituitary axis: transcribing the hypophysis.  |  Kioussi, C., et al. 1999. Mech Dev. 81: 23-35. PMID: 10330482
3. Limb development: Farewell to arms.  |  Graham, A. and McGonnell, I. 1999. Curr Biol. 9: R368-70. PMID: 10339420
4. Pitx2 regulates lung asymmetry, cardiac positioning and pituitary and tooth morphogenesis.  |  Lin, CR., et al. 1999. Nature. 401: 279-82. PMID: 10499586
5. Xpitx-1: a homeobox gene expressed during pituitary and cement gland formation of Xenopus embryos.  |  Hollemann, T. and Pieler, T. 1999. Mech Dev. 88: 249-52. PMID: 10534625
6. PITX1 promotes chondrogenesis and myogenesis in mouse hindlimbs through conserved regulatory targets.  |  Wang, JS., et al. 2018. Dev Biol. 434: 186-195. PMID: 29273440
7. Downregulated PITX1 Modulated by MiR-19a-3p Promotes Cell Malignancy and Predicts a Poor Prognosis of Gastric Cancer by Affecting Transcriptionally Activated PDCD5.  |  Qiao, F., et al. 2018. Cell Physiol Biochem. 46: 2215-2231. PMID: 29734189
8. Bioinformatics analysis of prognostic value of PITX1 gene in breast cancer.  |  Wang, Q., et al. 2020. Biosci Rep. 40: PMID: 32830857
9. The circ-PITX1 promotes non-small cell lung cancer development via the miR-30e-5p/ITGA6 axis.  |  Li, W., et al. 2022. Cell Cycle. 21: 304-321. PMID: 35007184
10. PITX1 suppresses osteosarcoma metastasis through exosomal LINC00662-mediated M2 macrophage polarization.  |  Zhang, Y., et al. 2023. Clin Exp Metastasis. 40: 79-93. PMID: 36334221
11. Pituitary homeobox 2, a novel member of the bicoid-related family of homeobox genes, is a potential regulator of anterior structure formation.  |  Gage, PJ. and Camper, SA. 1997. Hum Mol Genet. 6: 457-64. PMID: 9147650
12. Human and murine PTX1/Ptx1 gene maps to the region for Treacher Collins syndrome.  |  Crawford, MJ., et al. 1997. Mamm Genome. 8: 841-5. PMID: 9337397

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Pitx1 Antibody (G-4) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-Pitx1 Antibody (G-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Pitx1 Antibody (G-4) Specifications

Alternate Product Name: Pitx1

Product Clone Name: G-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 2-32

Epitope Target: Pitx1

Epitope Species: human

Product Reactivity/Detection: Pitx1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for Pitx1 Antibody (G-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent