PITPβ Antibody (G-3): sc-390500
- PITPβ Antibody (G-3) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 94-163 mapping within an internal region of PITPβ of human origin
- Anti-PITP beta Antibody (G-3) is recommended for detection of PITPβ of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-PITP beta Antibody (G-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for PITPβ Antibody (G-3). This work is in progress.
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PITPβ Antibody (G-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects PITPβ in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PITPβ (G-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PITPβ plays a crucial role in lipid metabolism by facilitating the transfer of phosphatidylinositol and phosphatidylcholine between cellular membranes, which is essential for generating second messenger molecules involved in critical signaling pathways such as phospholipase C and phosphoinositide 3-kinase. This transfer is vital for maintaining cellular functions, including membrane dynamics and signal transduction. PITPβ is a 271 amino acid protein widely expressed across various tissues and exists in two isoforms due to alternative splicing. While PITPβ is necessary for proper Golgi targeting, constitutive phosphorylation of Ser 262 does not influence phospholipid transfer activity, highlighting complex regulatory mechanisms. PITPβ interaction with other lipid-binding proteins in mammalian cells underscores importance in cellular homeostasis and signaling.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PITPβ Antibody (G-3) References:
- Mammalian phosphatidylinositol transfer proteins: emerging roles in signal transduction and vesicular traffic. | Cockcroft, S. 1999. Chem Phys Lipids. 98: 23-33. PMID: 10358925
- Phosphatidylinositol transfer protein beta displays minimal sphingomyelin transfer activity and is not required for biosynthesis and trafficking of sphingomyelin. | Ségui, B., et al. 2002. Biochem J. 366: 23-34. PMID: 12023904
- Structure of PITPbeta in complex with phosphatidylcholine: comparison of structure and lipid transfer to other PITP isoforms. | Vordtriede, PB., et al. 2005. Biochemistry. 44: 14760-71. PMID: 16274224
- Specific and nonspecific membrane-binding determinants cooperate in targeting phosphatidylinositol transfer protein beta-isoform to the mammalian trans-Golgi network. | Phillips, SE., et al. 2006. Mol Biol Cell. 17: 2498-512. PMID: 16540520
- Biochemical and biological functions of class I phosphatidylinositol transfer proteins. | Cockcroft, S. and Carvou, N. 2007. Biochim Biophys Acta. 1771: 677-91. PMID: 17490911
- Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer. | Rikova, K., et al. 2007. Cell. 131: 1190-203. PMID: 18083107
- Dynamics of lipid transfer by phosphatidylinositol transfer proteins in cells. | Shadan, S., et al. 2008. Traffic. 9: 1743-56. PMID: 18636990
- Cloning and expression of human cDNA encoding phosphatidylinositol transfer protein beta. | Tanaka, S., et al. 1995. Biochim Biophys Acta. 1259: 199-202. PMID: 8541325
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PITPβ Antibody (G-3) | sc-390500 | 200 µg/ml | $316.00 | |||
PITPβ Antibody (G-3) AC | sc-390500 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PITPβ Antibody (G-3) HRP | sc-390500 HRP | 200 µg/ml | $316.00 | |||
PITPβ Antibody (G-3) FITC | sc-390500 FITC | 200 µg/ml | $330.00 | |||
PITPβ Antibody (G-3) PE | sc-390500 PE | 200 µg/ml | $343.00 | |||
PITPβ Antibody (G-3) Alexa Fluor® 488 | sc-390500 AF488 | 200 µg/ml | $357.00 | |||
PITPβ Antibody (G-3) Alexa Fluor® 546 | sc-390500 AF546 | 200 µg/ml | $357.00 | |||
PITPβ Antibody (G-3) Alexa Fluor® 594 | sc-390500 AF594 | 200 µg/ml | $357.00 | |||
PITPβ Antibody (G-3) Alexa Fluor® 647 | sc-390500 AF647 | 200 µg/ml | $357.00 | |||
PITPβ Antibody (G-3) Alexa Fluor® 680 | sc-390500 AF680 | 200 µg/ml | $357.00 | |||
PITPβ Antibody (G-3) Alexa Fluor® 790 | sc-390500 AF790 | 200 µg/ml | $357.00 |