PITPα Antibody (5F12): sc-13569

PITPα Antibody (5F12) is a mouse monoclonal IgG1 kappa light chain antibody that detects the PITP alpha protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-PITPα antibody (5F12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PITP alpha protein plays a crucial role in lipid signaling by facilitating the transfer of phosphatidylinositol (PI) and phosphatidylcholine (PC) between cellular membranes, which is essential for the generation of second messenger molecules involved in critical signaling pathways such as phospholipase C and phosphoinositide 3-kinase. PITP alpha protein is predominantly located in the cytosol and is vital for maintaining cellular lipid homeostasis, as PITP alpha mobilizes PI from the endoplasmic reticulum and regulates PI release from vesicles in the Golgi network. PITP alpha, along with its isoforms, underscores its importance in various cellular processes, including membrane trafficking and signal transduction, making PITP alpha a significant target for research in cellular biology and disease mechanisms.

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PITPα Antibody (5F12) References:

1. Mammalian phosphatidylinositol transfer proteins: emerging roles in signal transduction and vesicular traffic.  |  Cockcroft, S. 1999. Chem Phys Lipids. 98: 23-33. PMID: 10358925
2. Involvement of PITPnm, a mammalian homologue of Drosophila rdgB, in phosphoinositide synthesis on Golgi membranes.  |  Aikawa, Y., et al. 1999. J Biol Chem. 274: 20569-77. PMID: 10400687
3. Cloning and characterization of a novel human phosphatidylinositol transfer protein, rdgBbeta.  |  Fullwood, Y., et al. 1999. J Biol Chem. 274: 31553-8. PMID: 10531358
4. Developmental changes in phosphatidylinositol transfer protein concentration and phospholipid transfer activities in rat type II cells.  |  Viscardi, RM. and Strauss, KA. 1999. Exp Lung Res. 25: 561-76. PMID: 10598318
5. Phosphatidylinositol transfer proteins couple lipid transport to phosphoinositide synthesis.  |  Cockcroft, S. 2001. Semin Cell Dev Biol. 12: 183-91. PMID: 11292384
6. RdgB proteins: functions in lipid homeostasis and signal transduction.  |  Trivedi, D. and Padinjat, R. 2007. Biochim Biophys Acta. 1771: 692-9. PMID: 17543578
7. The diverse functions of phosphatidylinositol transfer proteins.  |  Cockcroft, S. 2012. Curr Top Microbiol Immunol. 362: 185-208. PMID: 23086419
8. Courier service for phosphatidylinositol: PITPs deliver on demand.  |  Ashlin, TG., et al. 2021. Biochim Biophys Acta Mol Cell Biol Lipids. 1866: 158985. PMID: 34111527
9. Individual phosphatidylinositol transfer proteins have distinct functions that do not involve lipid transfer activity.  |  Zhao, L., et al. 2023. Blood Adv. 7: 4233-4246. PMID: 36930803
10. Microcolin H, a novel autophagy inducer, exerts potent antitumour activity by targeting PITPα/β.  |  Yang, H., et al. 2023. Signal Transduct Target Ther. 8: 428. PMID: 37963877
11. Cloning and characterization of human homologue of Drosophila retinal degeneration B: a candidate gene for degenerative retinal diseases.  |  Guo, J. and Yu, FX. 1997. Dev Genet. 20: 235-45. PMID: 9216063
12. Evidence that mammalian phosphatidylinositol transfer protein regulates phosphatidylcholine metabolism.  |  Monaco, ME., et al. 1998. Biochem J. 335 (Pt 1): 175-9. PMID: 9742227

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PITPα Antibody (5F12) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-PITPα Antibody (5F12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PITPα Antibody (5F12) Specifications

Alternate Product Name: PITPα

Product Clone Name: 5F12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: PITP

Epitope Species: human

Product Reactivity/Detection: PITPα

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for PITPα Antibody (5F12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred PITPα/β antibody is PITPα/β Antibody (B-9): sc-398050.