PITPα/β Antibody (B-9): sc-398050

PITPα/β Antibody (B-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects PITP alpha/beta protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PITPα/β (B-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The phosphatidylinositol transfer proteins (PITP) play a crucial role in cellular signaling by facilitating the transfer of phosphatidylinositol (PI) and phosphatidylcholine (PC) between membranes, which is essential for generating second messenger molecules involved in key signaling pathways such as phospholipase C and phosphoinositide 3-kinase. PITP proteins are ubiquitously expressed and are vital for mobilizing PI from the endoplasmic reticulum and regulating PI release from vesicles in the Golgi network, thereby influencing various cellular processes including membrane trafficking and signal transduction. Notably, the gene encoding human retinal degeneration B beta (rdgB beta) protein, which is one of the smaller soluble forms of PITP, is located on chromosome 11q13, a region associated with several inherited retinal degenerative diseases, highlighting PITP′s importance in both normal cellular function and disease pathology.

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PITPα/β Antibody (B-9) References:

1. Mammalian phosphatidylinositol transfer proteins: emerging roles in signal transduction and vesicular traffic.  |  Cockcroft, S. 1999. Chem Phys Lipids. 98: 23-33. PMID: 10358925
2. Involvement of PITPnm, a mammalian homologue of Drosophila rdgB, in phosphoinositide synthesis on Golgi membranes.  |  Aikawa, Y., et al. 1999. J Biol Chem. 274: 20569-77. PMID: 10400687
3. Cloning and characterization of a novel human phosphatidylinositol transfer protein, rdgBbeta.  |  Fullwood, Y., et al. 1999. J Biol Chem. 274: 31553-8. PMID: 10531358
4. Developmental changes in phosphatidylinositol transfer protein concentration and phospholipid transfer activities in rat type II cells.  |  Viscardi, RM. and Strauss, KA. 1999. Exp Lung Res. 25: 561-76. PMID: 10598318
5. Phosphatidylinositol transfer proteins couple lipid transport to phosphoinositide synthesis.  |  Cockcroft, S. 2001. Semin Cell Dev Biol. 12: 183-91. PMID: 11292384
6. The structure of phosphatidylinositol transfer protein alpha reveals sites for phospholipid binding and membrane association with major implications for its function.  |  van Tiel, CM., et al. 2002. FEBS Lett. 531: 69-73. PMID: 12401206
7. Phosphatidylinositol transfer proteins: emerging roles in cell proliferation, cell death and survival.  |  Snoek, GT. 2004. IUBMB Life. 56: 467-75. PMID: 15545226
8. Biochemical and biological functions of class I phosphatidylinositol transfer proteins.  |  Cockcroft, S. and Carvou, N. 2007. Biochim Biophys Acta. 1771: 677-91. PMID: 17490911
9. Potential role for phosphatidylinositol transfer protein (PITP) family in lipid transfer during phospholipase C signalling.  |  Cockcroft, S. and Garner, K. 2013. Adv Biol Regul. 53: 280-91. PMID: 23916246
10. Synthesis and Cellular Labeling of Caged Phosphatidylinositol Derivatives.  |  Müller, R., et al. 2020. Chemistry. 26: 384-389. PMID: 31550056
11. Cloning and characterization of human homologue of Drosophila retinal degeneration B: a candidate gene for degenerative retinal diseases.  |  Guo, J. and Yu, FX. 1997. Dev Genet. 20: 235-45. PMID: 9216063
12. Evidence that mammalian phosphatidylinositol transfer protein regulates phosphatidylcholine metabolism.  |  Monaco, ME., et al. 1998. Biochem J. 335 (Pt 1): 175-9. PMID: 9742227

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PITPα/β Antibody (B-9) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-PITPα/β Antibody (B-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PITPα/β Antibody (B-9) Specifications

Alternate Product Name: PITPα/β

Product Clone Name: B-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-270

Epitope Target: PITPα

Epitope Species: human

Product Reactivity/Detection: PITPα and PITPβ

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for PITPα/β Antibody (B-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred PITPα antibody is PITPα Antibody (5F12): sc-13569.