PIPPIN Antibody (G-4): sc-376693
- PIPPIN Antibody (G-4) is a mouse monoclonal IgG1 κ PIPPIN antibody provided at 200 µg/ml
- raised against amino acids 11-50 mapping near the N-terminus of PIPPIN of human origin
- PIPPIN Antibody (G-4) is recommended for detection of PIPPIN of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-PIPPIN Antibody (G-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for PIPPIN Antibody (G-4) for WB applications. This reagent is now offered in a bundle with PIPPIN Antibody (G-4) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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PIPPIN Antibody (G-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects PIPPIN protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PIPPIN (G-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PIPPIN protein, also known as CSDC2 (cold shock domain containing C2, RNA binding), is a member of the Y-box protein family, which consists of highly conserved RNA-binding transcriptional regulators. Predominantly expressed in brain cells, PIPPIN is localized in both the nucleus and cytoplasm, where PIPPIN plays a crucial role in regulating gene expression. PIPPIN features two RNA-binding motifs, PIP1 and PIP2, along with a cold shock domain, which are essential for function as a nucleic acid binding regulatory factor. Notably, PIPPIN is involved in brain maturation by binding to the 3′-untranslated regions of mRNAs encoding Histone H1 and Histone H3.3, a process that requires coordinated action of all PIPPIN domains. Additionally, PIPPIN can undergo sumoylation in a thyroid hormone (T3)-dependent manner, indicating that post-translational modifications may influence regulatory role in protein synthesis in response to extracellular signals.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PIPPIN Antibody (G-4) References:
- PIPPin is a brain-specific protein that contains a cold-shock domain and binds specifically to H1 degrees and H3.3 mRNAs. | Nastasi, T., et al. 1999. J Biol Chem. 274: 24087-93. PMID: 10446180
- Specific neurons of brain cortex and cerebellum are PIPPin positive. | Nastasi, T., et al. 2000. Neuroreport. 11: 2233-6. PMID: 10923677
- RNA-binding ability of PIPPin requires the entire protein. | Raimondi, L., et al. 2003. J Cell Mol Med. 7: 35-42. PMID: 12767259
- CRHSP-24 phosphorylation is regulated by multiple signaling pathways in pancreatic acinar cells. | Schäfer, C., et al. 2003. Am J Physiol Gastrointest Liver Physiol. 285: G726-34. PMID: 12801884
- Analysis of cytochrome C oxidase subunits III and IV expression in developing rat brain. | Cannino, G., et al. 2004. Neuroscience. 128: 91-8. PMID: 15450356
- Identification of calcium-regulated heat-stable protein of 24 kDa (CRHSP24) as a physiological substrate for PKB and RSK using KESTREL. | Auld, GC., et al. 2005. Biochem J. 389: 775-83. PMID: 15910284
- Thyroid hormones induce sumoylation of the cold shock domain-containing protein PIPPin in developing rat brain and in cultured neurons. | Bono, E., et al. 2007. Endocrinology. 148: 252-7. PMID: 17053029
- MicroRNA-373 induces expression of genes with complementary promoter sequences. | Place, RF., et al. 2008. Proc Natl Acad Sci U S A. 105: 1608-13. PMID: 18227514
- PIPPin, a putative RNA-binding protein specifically expressed in the rat brain. | Castiglia, D., et al. 1996. Biochem Biophys Res Commun. 218: 390-4. PMID: 8573167
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PIPPIN Antibody (G-4) | sc-376693 | 200 µg/ml | $322.00 | |||
PIPPIN Antibody (G-4): m-IgGκ BP-HRP Bundle | sc-523048 | 200 µg Ab, 40 µg BP | $361.00 | |||
PIPPIN Antibody (G-4) AC | sc-376693 AC | 500 µg/ml, 25% agarose | $424.00 | |||
PIPPIN Antibody (G-4) HRP | sc-376693 HRP | 200 µg/ml | $322.00 | |||
PIPPIN Antibody (G-4) FITC | sc-376693 FITC | 200 µg/ml | $336.00 | |||
PIPPIN Antibody (G-4) PE | sc-376693 PE | 200 µg/ml | $349.00 | |||
PIPPIN Antibody (G-4) Alexa Fluor® 488 | sc-376693 AF488 | 200 µg/ml | $364.00 | |||
PIPPIN Antibody (G-4) Alexa Fluor® 546 | sc-376693 AF546 | 200 µg/ml | $364.00 | |||
PIPPIN Antibody (G-4) Alexa Fluor® 594 | sc-376693 AF594 | 200 µg/ml | $364.00 | |||
PIPPIN Antibody (G-4) Alexa Fluor® 647 | sc-376693 AF647 | 200 µg/ml | $364.00 | |||
PIPPIN Antibody (G-4) Alexa Fluor® 680 | sc-376693 AF680 | 200 µg/ml | $364.00 | |||
PIPPIN Antibody (G-4) Alexa Fluor® 790 | sc-376693 AF790 | 200 µg/ml | $364.00 |