PIPK I α Antibody (D-12): sc-377021

PIPK I α Antibody (D-12) is a mouse monoclonal IgG2b kappa light chain antibody that detects PIPK I alpha protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). PIPK I α (D-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The phosphatidylinositol-4-phosphate-5-kinase (PIPK) family plays a crucial role in synthesizing phosphatidylinositol-4,5-bisphosphate, a key lipid that regulates essential cellular processes such as cell proliferation, survival, membrane trafficking, and cytoskeletal organization. Within this family, PIPK I α, along with isoforms PIPK I β and PIPK I γ, phosphorylates phosphatidylinositol 4-phosphate at the 5-hydroxyl position, which is vital for enzymatic activity and cellular localization. PIPK I α shows predominant expression in brain tissue, highlighting importance in neural functions and signaling pathways. The distinct activation loop present in PIPK family members determines substrate specificity and subcellular targeting, making these proteins integral to various signaling cascades. Understanding PIPK I α and its interactions can provide insights into contributions to cellular dynamics and potential implications in neurobiology and disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PIPK I α Antibody (D-12) References:

1. The activation loop of phosphatidylinositol phosphate kinases determines signaling specificity.  |  Kunz, J., et al. 2000. Mol Cell. 5: 1-11. PMID: 10678164
2. Autophosphorylation of type I phosphatidylinositol phosphate kinase regulates its lipid kinase activity.  |  Itoh, T., et al. 2000. J Biol Chem. 275: 19389-94. PMID: 10777481
3. A nuclear phosphoinositide kinase complex regulates p53.  |  Choi, S., et al. 2019. Nat Cell Biol. 21: 462-475. PMID: 30886346
4. PIP5K1A: a potential target for cancers with KRAS or TP53 mutations.  |  East, MP., et al. 2020. Nat Rev Drug Discov. 19: 436. PMID: 32332925
5. CircRNA PIP5K1A promotes the progression of glioma through upregulation of the TCF12/PI3K/AKT pathway by sponging miR-515-5p.  |  Zheng, K., et al. 2021. Cancer Cell Int. 21: 27. PMID: 33413401
6. Metabolic Reprogramming of NK Cells by Black Phosphorus Quantum Dots Potentiates Cancer Immunotherapy.  |  He, L., et al. 2023. Adv Sci (Weinh). 10: e2202519. PMID: 36683155
7. Circular RNA PIP5K1A Promotes Glucose and Lipid Metabolism Disorders and Inflammation in Type 2 Diabetes Mellitus.  |  Song, G., et al. 2024. Mol Biotechnol. 66: 3549-3558. PMID: 37966664
8. The cloning and sequence of the C isoform of PtdIns4P 5-kinase.  |  Divecha, N., et al. 1995. Biochem J. 309 (Pt 3): 715-9. PMID: 7639683
9. Type I phosphatidylinositol-4-phosphate 5-kinases are distinct members of this novel lipid kinase family.  |  Loijens, JC. and Anderson, RA. 1996. J Biol Chem. 271: 32937-43. PMID: 8955136
10. Type I phosphatidylinositol-4-phosphate 5-kinases. Cloning of the third isoform and deletion/substitution analysis of members of this novel lipid kinase family.  |  Ishihara, H., et al. 1998. J Biol Chem. 273: 8741-8. PMID: 9535851
11. Type I phosphatidylinositol-4-phosphate 5-kinases synthesize the novel lipids phosphatidylinositol 3,5-bisphosphate and phosphatidylinositol 5-phosphate.  |  Tolias, KF., et al. 1998. J Biol Chem. 273: 18040-6. PMID: 9660759
12. Structure of type IIbeta phosphatidylinositol phosphate kinase: a protein kinase fold flattened for interfacial phosphorylation.  |  Rao, VD., et al. 1998. Cell. 94: 829-39. PMID: 9753329

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PIPK I α Antibody (D-12) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-PIPK I α Antibody (D-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PIPK I α Antibody (D-12) Specifications

Alternate Product Name: PIPK I α

Product Clone Name: D-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 481-519

Epitope Target: PIPK I α

Epitope Species: mouse

Product Reactivity/Detection: PIPK I α (designated PIPK I β in mouse)

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for PIPK I α Antibody (D-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred PIPK I antibody is PIPK I Antibody (D-12): sc-365238.