PIP5KIII Antibody (64-Q6): sc-100408

PIP5KIII Antibody (64-Q6) is a mouse monoclonal IgG2a kappa light chain antibody that detects PIP5KIII protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-PIP5KIII antibody (64-Q6) is available as the non-conjugated form. PIP5KIII protein, also known as Phosphatidylinositol 3-phosphate 5-kinase, FAB1, CFD, PIKFYVE, or ZFYVE29, is a crucial enzyme that synthesizes phosphatidylinositol-3,5-bisphosphate by catalyzing the phosphorylation of phosphatidylinositol 3-phosphate at the fifth hydroxyl of the inositol ring. This phosphorylation is vital for various cellular processes, including the regulation of cytoskeletal functions, membrane trafficking, and receptor signaling, as this activity facilitates the recruitment of protein complexes to cell and endosomal membranes. PIP5KIII is composed of 2,098 amino acids and features a DEP domain, a FYVE-type zinc finger, and a PIPK domain, which are essential for function and interactions within the cell. Notably, PIP5KIII exists as four alternatively spliced isoforms, allowing for diverse functional roles in cellular dynamics. Defects in the gene encoding PIP5KIII are associated with corneal fleck dystrophy, an autosomal disorder characterized by the presence of small white flecks throughout the corneal stroma, highlighting PIP5KIII′s significance in maintaining cellular integrity and function.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PIP5KIII Antibody (64-Q6) References:

1. Phosphatidylinositol 3-phosphate-interacting domains in PIKfyve. Binding specificity and role in PIKfyve. Endomenbrane localization.  |  Sbrissa, D., et al. 2002. J Biol Chem. 277: 6073-9. PMID: 11706043
2. PIKfyve controls fluid phase endocytosis but not recycling/degradation of endocytosed receptors or sorting of procathepsin D by regulating multivesicular body morphogenesis.  |  Ikonomov, OC., et al. 2003. Mol Biol Cell. 14: 4581-91. PMID: 14551253
3. Hypertonic stress increases phosphatidylinositol 4,5-bisphosphate levels by activating PIP5KIbeta.  |  Yamamoto, M., et al. 2006. J Biol Chem. 281: 32630-8. PMID: 16943196
4. The mammalian phosphatidylinositol 3-phosphate 5-kinase (PIKfyve) regulates endosome-to-TGN retrograde transport.  |  Rutherford, AC., et al. 2006. J Cell Sci. 119: 3944-57. PMID: 16954148
5. The phosphoinositide kinase PIKfyve mediates epidermal growth factor receptor trafficking to the nucleus.  |  Kim, J., et al. 2007. Cancer Res. 67: 9229-37. PMID: 17909029
6. PIKfyve: Partners, significance, debates and paradoxes.  |  Shisheva, A. 2008. Cell Biol Int. 32: 591-604. PMID: 18304842
7. Elevated levels of PtdIns5P in NPM-ALK transformed cells: implication of PIKfyve.  |  Coronas, S., et al. 2008. Biochem Biophys Res Commun. 372: 351-5. PMID: 18501703
8. Loss of PIP5KIbeta demonstrates that PIP5KI isoform-specific PIP2 synthesis is required for IP3 formation.  |  Wang, Y., et al. 2008. Proc Natl Acad Sci U S A. 105: 14064-9. PMID: 18772378
9. PIKfyve regulation of endosome-linked pathways.  |  de Lartigue, J., et al. 2009. Traffic. 10: 883-93. PMID: 19582903
10. A novel PIKFYVE mutation in fleck corneal dystrophy.  |  Kotoulas, A., et al. 2011. Mol Vis. 17: 2776-81. PMID: 22065932
11. Production of phosphatidylinositol 5-phosphate via PIKfyve and MTMR3 regulates cell migration.  |  Oppelt, A., et al. 2013. EMBO Rep. 14: 57-64. PMID: 23154468
12. PIP5KIβ selectively modulates apical endocytosis in polarized renal epithelial cells.  |  Szalinski, CM., et al. 2013. PLoS One. 8: e53790. PMID: 23342003

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PIP5KIII Antibody (64-Q6) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-PIP5KIII Antibody (64-Q6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PIP5KIII Antibody (64-Q6) Specifications

Alternate Product Name: PIP5KIII

Product Clone Name: 64-Q6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 342-452

Epitope Target: PIP5KIII

Epitope Species: human

Product Reactivity/Detection: PIP5KIII

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for PIP5KIII Antibody (64-Q6): N/A