PINX1 Antibody (D-3): sc-374113
- PINX1 Antibody (D-3) is a mouse monoclonal IgG2b κ PINX1 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 1-150 mapping at the N-terminus of PINX1 of human origin
- PINX1 Antibody (D-3) is recommended for detection of PINX1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-PINX1 Antibody (D-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PINX1 Antibody (D-3) for WB applications. These reagents are now offered in bundles with PINX1 Antibody (D-3) (see ordering information below).
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PINX1 Antibody (D-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects PINX1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PINX1 (D-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PINX1, also known as LPTL or LPTS (liver-related putative tumor suppressor), plays a crucial role in telomere regulation and is primarily localized in nucleoli and telomere speckles. PINX1 contains a G-patch domain and a telomeric inhibiting domain (TID) at its C-terminus, which is essential for interaction with telomere protein TRF1 and telomerase reverse transcriptase TERT. The TID domain specifically binds to TERT, inhibiting TERT activity and thereby regulating telomerase function. This inhibition is vital as overexpression of PINX1 can lead to shortened telomeres, indicating PINX1′s role as a tumor suppressor by preventing excessive cell proliferation. Furthermore, PINX1 depletion significantly enhances telomerase activity, which may contribute to the tumorigenicity of cancer cells. In addition to telomere maintenance, PINX1 is also involved in the maturation of nucleolar RNA, highlighting PINX1′s multifaceted functions within the cell.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PINX1 Antibody (D-3) References:
- The Pin2/TRF1-interacting protein PinX1 is a potent telomerase inhibitor. | Zhou, XZ. and Lu, KP. 2001. Cell. 107: 347-59. PMID: 11701125
- The yeast homolog of human PinX1 is involved in rRNA and small nucleolar RNA maturation, not in telomere elongation inhibition. | Guglielmi, B. and Werner, M. 2002. J Biol Chem. 277: 35712-9. PMID: 12107183
- Human PinX1, a potent telomerase inhibitor, is not involved in human gastrointestinal tract carcinoma. | Akiyama, Y., et al. 2004. Oncol Rep. 11: 871-4. PMID: 15010887
- Mutational analysis of PINX1 in hereditary prostate cancer. | Hawkins, GA., et al. 2004. Prostate. 60: 298-302. PMID: 15264240
- Characterization of interactions between PinX1 and human telomerase subunits hTERT and hTR. | Banik, SS. and Counter, CM. 2004. J Biol Chem. 279: 51745-8. PMID: 15381700
- Loss of heterozygosity and histone hypoacetylation of the PINX1 gene are associated with reduced expression in gastric carcinoma. | Kondo, T., et al. 2005. Oncogene. 24: 157-64. PMID: 15637589
- hTERT, the catalytic component of telomerase, is downregulated in the haematopoietic stem cells of patients with chronic myeloid leukaemia. | Campbell, LJ., et al. 2006. Leukemia. 20: 671-9. PMID: 16498395
- Conserved interactions of the splicing factor Ntr1/Spp382 with proteins involved in DNA double-strand break repair and telomere metabolism. | Herrmann, G., et al. 2007. Nucleic Acids Res. 35: 2321-32. PMID: 17389648
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PINX1 Antibody (D-3) | sc-374113 | 200 µg/ml | $322.00 | |||
PINX1 Antibody (D-3): m-IgGκ BP-HRP Bundle | sc-522627 | 200 µg Ab, 40 µg BP | $361.00 | |||
PINX1 Antibody (D-3): m-IgG2b BP-HRP Bundle | sc-549047 | 200 µg Ab; 10 µg BP | $361.00 | |||
PINX1 Antibody (D-3) AC | sc-374113 AC | 500 µg/ml, 25% agarose | $424.00 | |||
PINX1 Antibody (D-3) HRP | sc-374113 HRP | 200 µg/ml | $322.00 | |||
PINX1 Antibody (D-3) FITC | sc-374113 FITC | 200 µg/ml | $336.00 | |||
PINX1 Antibody (D-3) PE | sc-374113 PE | 200 µg/ml | $349.00 | |||
PINX1 Antibody (D-3) Alexa Fluor® 488 | sc-374113 AF488 | 200 µg/ml | $364.00 | |||
PINX1 Antibody (D-3) Alexa Fluor® 546 | sc-374113 AF546 | 200 µg/ml | $364.00 | |||
PINX1 Antibody (D-3) Alexa Fluor® 594 | sc-374113 AF594 | 200 µg/ml | $364.00 | |||
PINX1 Antibody (D-3) Alexa Fluor® 647 | sc-374113 AF647 | 200 µg/ml | $364.00 | |||
PINX1 Antibody (D-3) Alexa Fluor® 680 | sc-374113 AF680 | 200 µg/ml | $364.00 | |||
PINX1 Antibody (D-3) Alexa Fluor® 790 | sc-374113 AF790 | 200 µg/ml | $364.00 |