Pinin CRISPR/Cas9 KO Plasmid (h2): sc-405373-KO-2
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Pinin CRISPR/Cas9 Knockout (KO) Plasmid (h2) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Pinin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Pinin Antibody (4FQ): sc-101127
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Pinin CRISPR/Cas9 KO Plasmid (h2) | sc-405373-KO-2 | 20 µg | $397.00 |
Overview
PNN encodes pinin, a predominantly nuclear protein enriched at desmosome-associated complexes and within speckle-like domains where it couples cell–cell adhesion status to nuclear organization. Pinin participates in pre-mRNA processing by interacting with splicing regulators and components of the exon junction complex, thereby influencing alternative splicing programs linked to epithelial identity and differentiation. Through these roles, PNN impacts transcriptional and post-transcriptional control of genes governing proliferation, migration, and stress responses. Dysregulated PNN expression or splicing networks has been reported across multiple tumor contexts, supporting its utility in studying epithelial–mesenchymal dynamics and RNA-processing–driven phenotypes.
Pinin CRISPR/Cas9 KO Plasmid (h2) is a pool of plasmids designed for targeted disruption of the PNN gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PNN together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PNN open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Pinin protein expression.
This CRISPR knockout system enables efficient generation of PNN-deficient cell models for investigation of Pinin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PNN exon(s) critical for Pinin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PNN genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Pinin CRISPR/Cas9 KO Plasmid (h) and Pinin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PNN locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Pinin HDR Plasmid (h) and Pinin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PNN homology arms to support homology-directed repair at defined PNN target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.