Pin1 Antibody (G-8): sc-46660
- Pin1 Antibody (G-8) is a mouse monoclonal IgG2a κ Pin1 antibody, cited in 72 publications, provided at 200 µg/ml
- raised against amino acids 41-163 mapping at the C-terminus of Pin1 of human origin
- Pin1 Antibody (G-8) is recommended for detection of Pin1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Pin1 Antibody (G-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Pin1 Antibody (G-8) for WB and IHC(P) applications. These reagents are now offered in bundles with Pin1 Antibody (G-8) (see ordering information below).
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Pin1 Antibody (G-8) is a mouse monoclonal IgG2a kappa light chain antibody engineered to detect Pin1, also known as peptidylprolyl cis/trans isomerase, NIMA-interacting 1. Anti-Pin1 antibody (G-8) recognizes the C-terminal epitope comprising amino acids 41-163 of human Pin1, ensuring precise and reliable binding. Pin1 is a crucial enzyme that catalyzes the cis/trans isomerization of phosphorylated serine/threonine-proline motifs, a process essential for proper protein folding, function, and regulation of various signaling pathways. This isomerase activity is vital for cell cycle progression and has been implicated in numerous diseases, including cancer and neurodegenerative disorders, underscoring its importance in both normal cellular physiology and pathological conditions. Pin1 monoclonal antibody (G-8) demonstrates broad species reactivity, effectively detecting Pin1 from mouse, rat, and human origins, and is recommended for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). This versatility makes Pin1 (G-8) antibody an indispensable tool for researchers investigating the mechanistic roles of Pin1 in cell viability and disease mechanisms. Available in both non-conjugated form and multiple conjugated variants, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, Pin1 (G-8) monoclonal antibody accommodates diverse experimental requirements.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Pin1 Antibody (G-8) References:
- A mammalian dual specificity protein kinase, Nek1, is related to the NIMA cell cycle regulator and highly expressed in meiotic germ cells. | Letwin, K., et al. 1992. EMBO J. 11: 3521-31. PMID: 1382974
- Mitotic induction and maintenance by overexpression of a G2-specific gene that encodes a potential protein kinase. | Osmani, SA., et al. 1988. Cell. 53: 237-44. PMID: 3359487
- Cell cycle-dependent expression of Nek2, a novel human protein kinase related to the NIMA mitotic regulator of Aspergillus nidulans. | Schultz, SJ., et al. 1994. Cell Growth Differ. 5: 625-35. PMID: 7522034
- A human peptidyl-prolyl isomerase essential for regulation of mitosis. | Lu, KP., et al. 1996. Nature. 380: 544-7. PMID: 8606777
- The NIMA-related kinase 2, Nek2, is expressed in specific stages of the meiotic cell cycle and associates with meiotic chromosomes. | Rhee, K. and Wolgemuth, DJ. 1997. Development. 124: 2167-77. PMID: 9187143
- Structural and functional analysis of the mitotic rotamase Pin1 suggests substrate recognition is phosphorylation dependent. | Ranganathan, R., et al. 1997. Cell. 89: 875-86. PMID: 9200606
- Characterization of mammalian NIMA-related kinases. | Fry, AM. and Nigg, EA. 1997. Methods Enzymol. 283: 270-82. PMID: 9251026
- Sequence-specific and phosphorylation-dependent proline isomerization: a potential mitotic regulatory mechanism. | Yaffe, MB., et al. 1997. Science. 278: 1957-60. PMID: 9395400
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Pin1 Antibody (G-8) | sc-46660 | 200 µg/ml | $316.00 | |||
Pin1 Antibody (G-8): m-IgG Fc BP-HRP Bundle | sc-528492 | 200 µg Ab; 10 µg BP | $354.00 | |||
Pin1 Antibody (G-8): m-IgGκ BP-HRP Bundle | sc-520883 | 200 µg Ab, 40 µg BP | $354.00 | |||
Pin1 Antibody (G-8): m-IgG2a BP-HRP Bundle | sc-547107 | 200 µg Ab; 10 µg BP | $354.00 | |||
Pin1 Antibody (G-8) AC | sc-46660 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Pin1 Antibody (G-8) HRP | sc-46660 HRP | 200 µg/ml | $316.00 | |||
Pin1 Antibody (G-8) FITC | sc-46660 FITC | 200 µg/ml | $330.00 | |||
Pin1 Antibody (G-8) PE | sc-46660 PE | 200 µg/ml | $343.00 | |||
Pin1 Antibody (G-8) Alexa Fluor® 488 | sc-46660 AF488 | 200 µg/ml | $357.00 | |||
Pin1 Antibody (G-8) Alexa Fluor® 546 | sc-46660 AF546 | 200 µg/ml | $357.00 | |||
Pin1 Antibody (G-8) Alexa Fluor® 594 | sc-46660 AF594 | 200 µg/ml | $357.00 | |||
Pin1 Antibody (G-8) Alexa Fluor® 647 | sc-46660 AF647 | 200 µg/ml | $357.00 | |||
Pin1 Antibody (G-8) Alexa Fluor® 680 | sc-46660 AF680 | 200 µg/ml | $357.00 | |||
Pin1 Antibody (G-8) Alexa Fluor® 790 | sc-46660 AF790 | 200 µg/ml | $357.00 |