Pim-2 Antibody (1D12): sc-13514

Pim-2 Antibody (1D12) is a mouse monoclonal IgG1 kappa light chain antibody that detects Pim-2 of mouse, rat, and human origin by WB, IP, IF, and IHCP. Pim-2 (1D12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. Pim-2, also known as provirus integration site for Moloney murine leukemia virus, functions as a serine/threonine kinase that plays a critical role in cell survival and proliferation by promoting cell cycle progression and inhibiting apoptosis. This function is particularly important in hematopoietic cells, where Pim-2 is involved in the regulation of hematopoiesis and maintenance of stem cell populations. Pim-2 has been shown to interact with various proteins, including pro-apoptotic protein Bad, which Pim-2 phosphorylates to inhibit pro-apoptotic activity, thereby contributing to survival of cells under stress conditions. Human transcripts for Pim-2 have been detected in various tissues, including hematopoietic lineages, leukemic and lymphomic cells, as well as in brain, where Pim-2 is implicated in formation and preservation of long-term potentiation, a process essential for long-term memory.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Pim-2 Antibody (1D12) References:

1. Pim kinase expression is induced by LTP stimulation and required for the consolidation of enduring LTP.  |  Konietzko, U., et al. 1999. EMBO J. 18: 3359-69. PMID: 10369676
2. Developmental expression of pim kinases suggests functions also outside of the hematopoietic system.  |  Eichmann, A., et al. 2000. Oncogene. 19: 1215-24. PMID: 10713710
3. Lymphocyte transformation by Pim-2 is dependent on nuclear factor-kappaB activation.  |  Hammerman, PS., et al. 2004. Cancer Res. 64: 8341-8. PMID: 15548703
4. Pim and Akt oncogenes are independent regulators of hematopoietic cell growth and survival.  |  Hammerman, PS., et al. 2005. Blood. 105: 4477-83. PMID: 15705789
5. Antisense oligodeoxynucleotides targeting the serine/threonine kinase Pim-2 inhibited proliferation of DU-145 cells.  |  Dai, JM., et al. 2005. Acta Pharmacol Sin. 26: 364-8. PMID: 15715935
6. Integrating molecular dynamics simulation and molecular mechanics/generalized Born surface area calculation into pharmacophore modeling: a case study on the proviral integration site for Moloney murine leukemia virus (Pim)-1 kinase inhibitors.  |  Gao, J., et al. 2020. J Biomol Struct Dyn. 38: 581-588. PMID: 30678548
7. PIM1 (Provirus Integration Site For Moloney Murine Leukemia Virus) as a Novel Biomarker and Therapeutic Target in Pulmonary Arterial Hypertension: Another Evidence for Cancer Theory.  |  Satoh, K., et al. 2020. Arterioscler Thromb Vasc Biol. 40: 500-502. PMID: 32101474
8. B-cell-specific Moloney murine leukemia virus integration site 1 knockdown impairs adriamycin resistance of gastric cancer cells.  |  Ma, N., et al. 2023. Arab J Gastroenterol. 24: 168-174. PMID: 36878814
9. An XBP1s-PIM-2 positive feedback loop controls IL-15-mediated survival of natural killer cells.  |  Ma, S., et al. 2023. Sci Immunol. 8: eabn7993. PMID: 36897958
10. Proviral tagging in E mu-myc transgenic mice lacking the Pim-1 proto-oncogene leads to compensatory activation of Pim-2.  |  van der Lugt, NM., et al. 1995. EMBO J. 14: 2536-44. PMID: 7781606
11. Pim-2 transgene induces lymphoid tumors, exhibiting potent synergy with c-myc.  |  Allen, JD., et al. 1997. Oncogene. 15: 1133-41. PMID: 9294606
12. The human Pim-2 proto-oncogene and its testicular expression.  |  Baytel, D., et al. 1998. Biochim Biophys Acta. 1442: 274-85. PMID: 9804974

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Pim-2 Antibody (1D12) has 31 citations in a variety of scientific publications.

Primary antibodies like Anti-Pim-2 Antibody (1D12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Pim-2 Antibody (1D12) Specifications

Alternate Product Name: PIM2

Product Clone Name: 1D12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Pim-2

Epitope Species: mouse

Product Reactivity/Detection: Pim-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 31 citations in various scientific publications

Available Conjugates/Forms for Pim-2 Antibody (1D12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin