Pim-1 Antibody (12H8): sc-13513

Pim-1 Antibody (12H8) is a mouse monoclonal IgG1 kappa light chain antibody that detects Pim-1 in mouse, rat, and human samples through western blotting (WB) and immunoprecipitation (IP). Pim-1 (12H8) antibody is available in both conjugated and non-conjugated forms, providing versatility for various experimental needs. Pim-1 is a serine/threonine kinase that plays a crucial role in cell signaling pathways, particularly in lymphoid cell transformation, where Pim-1 collaborates with c-Myc to promote cell proliferation and survival. Pim-1 is predominantly located in the cytoplasm and nucleus, which is significant as Pim-1 localization is essential for function in regulating cell cycle and apoptosis. Specifically, Pim-1 expression increases during transition from early to late G1 phase, remaining elevated at G1/S boundary and G2 phases, thereby influencing cell cycle progression. Additionally, Pim-1 is regulated at both transcriptional and translational levels, with Pim-1 expression being induced by interleukin-2 stimulation, highlighting Pim-1′s importance in T cell differentiation and immune responses. Furthermore, Pim-1 has been shown to stimulate c-Myc-mediated apoptosis upstream of caspase-3-like proteases, underscoring Pim-1′s role in maintaining cellular homeostasis and preventing uncontrolled cell growth.

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Pim-1 Antibody (12H8) References:

1. Understanding the functional discrepancy of Pim-1 in cancer.  |  Ouhtit, A., et al. 2015. Front Biosci (Elite Ed). 7: 208-14. PMID: 25553374
2. PIM-1 may function as an oncogene in cervical cancer via activating the EGFR signaling.  |  Yang, H., et al. 2020. Int J Biol Markers. 35: 67-73. PMID: 32914663
3. Pim-1 kinase is a positive feedback regulator of the senescent lung fibroblast inflammatory secretome.  |  Gao, AY., et al. 2022. Am J Physiol Lung Cell Mol Physiol. 323: L685-L697. PMID: 36223640
4. Targeting macrophagic PIM-1 alleviates osteoarthritis by inhibiting NLRP3 inflammasome activation via suppressing mitochondrial ROS/Cl- efflux signaling pathway.  |  Zhang, Z., et al. 2023. J Transl Med. 21: 452. PMID: 37422640
5. Inhibition of DYRK1A attenuates vascular remodeling in pulmonary arterial hypertension via suppressing STAT3/Pim-1/NFAT pathway.  |  Lan, C., et al. 2024. Clin Exp Hypertens. 46: 2297642. PMID: 38147409
6. The role of Pim-1 kinases in inflammatory signaling pathways.  |  Baek, HS., et al. 2024. Inflamm Res. 73: 1671-1685. PMID: 39079978
7. Ubiquitous expression and cell cycle regulation of the protein kinase PIM-1.  |  Liang, H., et al. 1996. Arch Biochem Biophys. 330: 259-65. PMID: 8660654
8. The effect of IL-2 treatment on transcriptional attenuation in proto-oncogenes pim-1 and c-myb in human thymic blast cells.  |  Rohwer, F., et al. 1996. J Immunol. 157: 643-9. PMID: 8752912
9. Pim-1 kinase stimulates c-Myc-mediated death signaling upstream of caspase-3 (CPP32)-like protease activation.  |  Mochizuki, T., et al. 1997. Oncogene. 15: 1471-80. PMID: 9333023
10. Pim-1 protein expression is regulated by its 5'-untranslated region and translation initiation factor elF-4E.  |  Hoover, DS., et al. 1997. Cell Growth Differ. 8: 1371-80. PMID: 9419425
11. Evidence implicating Gfi-1 and Pim-1 in pre-T-cell differentiation steps associated with beta-selection.  |  Schmidt, T., et al. 1998. EMBO J. 17: 5349-59. PMID: 9736613
12. Pim-1 kinase and p100 cooperate to enhance c-Myb activity.  |  Leverson, JD., et al. 1998. Mol Cell. 2: 417-25. PMID: 9809063

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Pim-1 Antibody (12H8) has 99 citations in a variety of scientific publications.

Primary antibodies like Anti-Pim-1 Antibody (12H8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Pim-1 Antibody (12H8) Specifications

Alternate Product Name: Pim-1

Product Clone Name: 12H8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Pim-1

Epitope Species: mouse

Product Reactivity/Detection: Pim-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 99 citations in various scientific publications

Available Conjugates/Forms for Pim-1 Antibody (12H8): AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790

The preferred Pim-1 antibody is Pim-1 Antibody (G-11): sc-374116.