Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PILR-α CRISPR/Cas9 KO Plasmid (h): sc-407310
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PILR-α CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PILR-α genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PILR-α Antibody (47-12): sc-100286
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PILR-α CRISPR/Cas9 KO Plasmid (h) | sc-407310 | 20 µg | $397.00 |
Overview
PILRA (paired immunoglobulin-like type 2 receptor alpha) encodes an inhibitory cell-surface receptor enriched on myeloid populations, where it recognizes sialylated O-glycan ligands and transduces suppressive signaling through ITIM-dependent recruitment of phosphatases such as SHP-1/2. By dampening Fc receptor- and TLR-associated activation programs, PILR-α contributes to regulation of innate immune thresholds, cytokine output, and leukocyte effector functions. This checkpoint-like activity links PILR-α to pathways governing inflammatory homeostasis, antigen-presenting cell activation, and immune evasion mechanisms. Altered PILRA expression or ligand engagement has been implicated in neuroinflammation and infectious disease biology, making it relevant for studies of myeloid regulation in complex tissue microenvironments.
PILR-α CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PILRA gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PILRA together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PILRA open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PILR-α protein expression.
This CRISPR knockout system enables efficient generation of PILRA-deficient cell models for investigation of PILR-α signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PILRA exon(s) critical for PILR-α function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PILRA genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PILR-α CRISPR/Cas9 KO Plasmid (h) and PILR-α CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PILRA locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PILR-α HDR Plasmid (h) and PILR-α HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PILRA homology arms to support homology-directed repair at defined PILRA target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.