Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PIKE CRISPR/Cas9 KO Plasmid (h): sc-405344
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PIKE CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PIKE genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PIKE Antibody (G-9): sc-166864
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PIKE CRISPR/Cas9 KO Plasmid (h) | sc-405344 | 20 µg | $397.00 |
Overview
AGAP2 encodes PIKE, a multidomain GTPase and signaling adaptor that couples growth factor receptor inputs to phosphoinositide 3-kinase (PI3K) signaling, thereby influencing PIP3-dependent pathways that regulate proliferation, survival, and cytoskeletal dynamics. PIKE interacts with components of receptor tyrosine kinase cascades and modulates downstream effectors such as AKT, impacting endocytosis, vesicle trafficking, and actin remodeling in multiple cell types. Dysregulated AGAP2/PIKE activity has been linked to altered oncogenic signaling, metabolic stress responses, and invasive phenotypes through perturbation of PI3K-centered network wiring. These properties make AGAP2 a useful node for dissecting signal integration and feedback control within growth and survival pathways in human cellular models.
PIKE CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the AGAP2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the AGAP2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the AGAP2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PIKE protein expression.
This CRISPR knockout system enables efficient generation of AGAP2-deficient cell models for investigation of PIKE signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting AGAP2 exon(s) critical for PIKE function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple AGAP2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PIKE CRISPR/Cas9 KO Plasmid (h) and PIKE CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the AGAP2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PIKE HDR Plasmid (h) and PIKE HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by AGAP2 homology arms to support homology-directed repair at defined AGAP2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.