PIKE Antibody (G-9): sc-166864

PIKE Antibody (G-9) is a mouse monoclonal IgG2a kappa light chain antibody that detects PIKE protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-PIKE antibody (G-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PIKE protein, also known as Phosphatidylinositol-3-kinase enhancer, CENTG1, AGAP2, or GGAP2, is a 1,192 amino acid protein that exists in two isoforms: PIKE-L, which is specific to the brain, and PIKE-A (or PIKE-S), which is distributed throughout the body. PIKE protein is localized in both the nucleus and cytoplasm, where PIKE functions as a GTPase-activating protein (GAP) activated by phosphatidylinositol 4,5-bisphosphate (PIP2), playing a crucial role in preventing neuronal apoptosis. PIKE protein interacts with several key proteins, including ARF1, ARF5, phospholipase C gamma 1, and Homer, thereby regulating endosomal trafficking and protein coupling events essential for cellular signaling. PIKE structural composition includes one pleckstrin homology (PH) domain, one Miro domain, one Arf-GAP domain, and two ankyrin repeats, which are vital for protein-binding and GAP activity. PIKE overexpression is linked to tumor growth and invasion, while reduced PIKE levels are associated with neuronal cell death, highlighting PIKE′s dual role in cancer biology and neuroprotection.

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PIKE Antibody (G-9) References:

1. Pike. A nuclear gtpase that enhances PI3kinase activity and is regulated by protein 4.1N.  |  Ye, K., et al. 2000. Cell. 103: 919-30. PMID: 11136977
2. AGAP1, an endosome-associated, phosphoinositide-dependent ADP-ribosylation factor GTPase-activating protein that affects actin cytoskeleton.  |  Nie, Z., et al. 2002. J Biol Chem. 277: 48965-75. PMID: 12388557
3. PIKE (phosphatidylinositol 3-kinase enhancer)-A GTPase stimulates Akt activity and mediates cellular invasion.  |  Ahn, JY., et al. 2004. J Biol Chem. 279: 16441-51. PMID: 14761976
4. PIKE-A is amplified in human cancers and prevents apoptosis by up-regulating Akt.  |  Ahn, JY., et al. 2004. Proc Natl Acad Sci U S A. 101: 6993-8. PMID: 15118108
5. PIKE GTPase signaling and function.  |  Ahn, JY. and Ye, K. 2005. Int J Biol Sci. 1: 44-50. PMID: 15951849
6. PIKE GTPase-mediated nuclear signalings promote cell survival.  |  Ye, K. 2006. Biochim Biophys Acta. 1761: 570-6. PMID: 16567124
7. PIKE-A is a proto-oncogene promoting cell growth, transformation and invasion.  |  Liu, X., et al. 2007. Oncogene. 26: 4918-27. PMID: 17297440
8. PIKE GTPase are phosphoinositide-3-kinase enhancers, suppressing programmed cell death.  |  Chan, CB. and Ye, K. 2007. J Cell Mol Med. 11: 39-53. PMID: 17367500
9. Neuroprotective actions of PIKE-L by inhibition of SET proteolytic degradation by asparagine endopeptidase.  |  Liu, Z., et al. 2008. Mol Cell. 29: 665-78. PMID: 18374643
10. Netrin-1 mediates neuronal survival through PIKE-L interaction with the dependence receptor UNC5B.  |  Tang, X., et al. 2008. Nat Cell Biol. 10: 698-706. PMID: 18469807
11. Cdk5-mediated regulation of the PIKE-A-Akt pathway and glioblastoma cell invasion.  |  Liu, R., et al. 2008. Proc Natl Acad Sci U S A. 105: 7570-5. PMID: 18487454
12. The roles of PIKE in tumorigenesis.  |  Qi, Q. and Ye, K. 2013. Acta Pharmacol Sin. 34: 991-7. PMID: 23770988

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PIKE Antibody (G-9) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-PIKE Antibody (G-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PIKE Antibody (G-9) Specifications

Alternate Product Name: PIKE

Product Clone Name: G-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 793-810

Epitope Target: PIKE

Epitope Species: mouse

Product Reactivity/Detection: PIKE

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for PIKE Antibody (G-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin