PIG-S Antibody (G-10): sc-373701

PIG-S Antibody (G-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects PIG-S protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PIG-S (G-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PIG-S protein plays a critical role in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors, which are essential for the proper localization and function of many membrane proteins. Located in the endoplasmic reticulum, PIG-S is a key component of the GPI transamidase (GPIT) complex, which recognizes C-terminal signal sequences on proproteins, cleaves them, and replaces them with specific GPI lipids. This process is vital for generating the carbonyl intermediate necessary for GPI anchor synthesis, thereby influencing the stability and functionality of various proteins that rely on GPI anchoring for their activity. PIG-S interaction with other proteins in the GPIT complex underscores its importance in cellular signaling and membrane dynamics, making anti-PIG-S antibody (G-10) an invaluable tool for researchers studying GPI biosynthesis and related pathways.

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PIG-S Antibody (G-10) References:

1. PIG-S and PIG-T, essential for GPI anchor attachment to proteins, form a complex with GAA1 and GPI8.  |  Ohishi, K., et al. 2001. EMBO J. 20: 4088-98. PMID: 11483512
2. Human PIG-U and yeast Cdc91p are the fifth subunit of GPI transamidase that attaches GPI-anchors to proteins.  |  Hong, Y., et al. 2003. Mol Biol Cell. 14: 1780-9. PMID: 12802054
3. GPI transamidase of Trypanosoma brucei has two previously uncharacterized (trypanosomatid transamidase 1 and 2) and three common subunits.  |  Nagamune, K., et al. 2003. Proc Natl Acad Sci U S A. 100: 10682-7. PMID: 12958211
4. A conserved proline in the last transmembrane segment of Gaa1 is required for glycosylphosphatidylinositol (GPI) recognition by GPI transamidase.  |  Vainauskas, S. and Menon, AK. 2004. J Biol Chem. 279: 6540-5. PMID: 14660601
5. Gpi17p does not stably interact with other subunits of glycosylphosphatidylinositol transamidase in Saccharomyces cerevisiae.  |  Zhu, Y., et al. 2005. Biochim Biophys Acta. 1735: 79-88. PMID: 15939668
6. TbGPI16 is an essential component of GPI transamidase in Trypanosoma brucei.  |  Hong, Y., et al. 2006. FEBS Lett. 580: 603-6. PMID: 16405969
7. Mutational analysis of the glycosylphosphatidylinositol (GPI) anchor pathway demonstrates that GPI-anchored proteins are required for cell wall biogenesis and normal hyphal growth in Neurospora crassa.  |  Bowman, SM., et al. 2006. Eukaryot Cell. 5: 587-600. PMID: 16524913
8. Biosynthesis and function of GPI proteins in the yeast Saccharomyces cerevisiae.  |  Pittet, M. and Conzelmann, A. 2007. Biochim Biophys Acta. 1771: 405-20. PMID: 16859984
9. Ethanolamine phosphate linked to the first mannose residue of glycosylphosphatidylinositol (GPI) lipids is a major feature of the GPI structure that is recognized by human GPI transamidase.  |  Vainauskas, S. and Menon, AK. 2006. J Biol Chem. 281: 38358-64. PMID: 17060324

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PIG-S Antibody (G-10) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-PIG-S Antibody (G-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PIG-S Antibody (G-10) Specifications

Alternate Product Name: PIG-S

Product Clone Name: G-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 199-231

Epitope Target: PIG-S

Epitope Species: human

Product Reactivity/Detection: PIG-S

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for PIG-S Antibody (G-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin