PICK1 Antibody (B-6): sc-365403

PICK1 Antibody (B-6) is a mouse monoclonal IgG2b antibody that detects PICK1 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PICK1, or protein interacting with C kinase 1, is a PDZ-domain containing protein predominantly located in the perinuclear region of cells, where PICK1 plays a crucial role in cellular signaling and synaptic transmission. The localization of PICK1 is significant as PICK1 allows for the precise regulation of interactions with other proteins, such as the α-amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid (AMPA) receptor, which is essential for excitatory synaptic transmission. By anchoring AMPA receptors at specific synapses, PICK1 modulates synaptic strength and plasticity, processes that are vital for learning and memory. Additionally, PICK1 is phosphorylated in response to protein kinase C alpha (PKCα) activation, which is critical for the regulation of cell proliferation and differentiation. The unique PDZ-binding domain of PKCα, absent in other PKC isoforms, facilitates the interaction with PICK1, highlighting PICK1′s importance in various signaling pathways. Anti-PICK1 antibody (B-6) is an invaluable tool for researchers studying these complex cellular mechanisms and their implications in neurobiology and other fields.

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PICK1 Antibody (B-6) References:

1. PICK1 targets activated protein kinase Calpha to AMPA receptor clusters in spines of hippocampal neurons and reduces surface levels of the AMPA-type glutamate receptor subunit 2.  |  Perez, JL., et al. 2001. J Neurosci. 21: 5417-28. PMID: 11466413
2. Lipid binding regulates synaptic targeting of PICK1, AMPA receptor trafficking, and synaptic plasticity.  |  Jin, W., et al. 2006. J Neurosci. 26: 2380-90. PMID: 16510715
3. PICK1 uncoupling from mGluR7a causes absence-like seizures.  |  Bertaso, F., et al. 2008. Nat Neurosci. 11: 940-8. PMID: 18641645
4. PICK1 is implicated in organelle motility in an Arp2/3 complex-independent manner.  |  Madasu, Y., et al. 2015. Mol Biol Cell. 26: 1308-22. PMID: 25657323
5. PICK1 confers anti-inflammatory effects in acute liver injury via suppressing M1 macrophage polarization.  |  Xie, J., et al. 2016. Biochimie. 127: 121-32. PMID: 27157267
6. Functional Regulation of Dopamine D₃ Receptor through Interaction with PICK1.  |  Zheng, M., et al. 2016. Biomol Ther (Seoul). 24: 475-81. PMID: 27169823
7. PICK1 Regulates the Expression and Trafficking of AMPA Receptors in Remifentanil-Induced Hyperalgesia.  |  Wang, Z., et al. 2016. Anesth Analg. 123: 771-81. PMID: 27537764
8. The TGF-β signalling negative regulator PICK1 represses prostate cancer metastasis to bone.  |  Dai, Y., et al. 2017. Br J Cancer. 117: 685-694. PMID: 28697177
9. Potent PDZ-Domain PICK1 Inhibitors that Modulate Amyloid Beta-Mediated Synaptic Dysfunction.  |  Lin, EYS., et al. 2018. Sci Rep. 8: 13438. PMID: 30194389
10. PICK1 attenuates high glucose-induced pancreatic β-cell death through the PI3K/Akt pathway and is negatively regulated by miR-139-5p.  |  Qiu, H., et al. 2020. Biochem Biophys Res Commun. 522: 14-20. PMID: 31735337

The preferred PICK1 antibody is PICK1 Antibody (D-10): sc-390479.