PICK1 Antibody (A-8): sc-166591

PICK1 Antibody (A-8) is a mouse monoclonal IgG2a light chain antibody that detects PICK1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PICK1, or protein interacting with C kinase 1, is a PDZ-domain containing protein predominantly located in the perinuclear region of cells, where PICK1 plays a crucial role in synaptic transmission and cellular signaling. The localization of PICK1 allows for precise regulation of interactions with other proteins, such as the α-amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid (AMPA) receptor, which is essential for excitatory synaptic transmission. By anchoring AMPA receptors at specific synapses, PICK1 modulates synaptic strength and plasticity, processes vital for learning and memory. Furthermore, PICK1 is phosphorylated in response to protein kinase C alpha (PKCα) activation, highlighting PICK1′s involvement in signaling pathways that regulate cell proliferation and differentiation. The unique PDZ-binding domain of PKCα, which is absent in other PKC isoforms, allows for specific interactions with PICK1, emphasizing PICK1′s importance in cellular communication and function. Anti-PICK1 antibody (A-8) is an invaluable tool for researchers studying these critical processes in various biological contexts.

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PICK1 Antibody (A-8) References:

1. Calcium binding to PICK1 is essential for the intracellular retention of AMPA receptors underlying long-term depression.  |  Citri, A., et al. 2010. J Neurosci. 30: 16437-52. PMID: 21147983
2. The BAR domain protein PICK1 controls vesicle number and size in adrenal chromaffin cells.  |  Pinheiro, PS., et al. 2014. J Neurosci. 34: 10688-700. PMID: 25100601
3. Syntabulin regulates the trafficking of PICK1-containing vesicles in neurons.  |  Xu, J., et al. 2016. Sci Rep. 6: 20924. PMID: 26868290
4. Binding of PICK1 PDZ domain with calcineurin B regulates osteoclast differentiation.  |  Kamano, Y., et al. 2018. Biochem Biophys Res Commun. 496: 83-88. PMID: 29305867
5. PICK1 deficiency exacerbates sepsis-associated acute lung injury and impairs glutathione synthesis via reduction of xCT.  |  Qian, M., et al. 2018. Free Radic Biol Med. 118: 23-34. PMID: 29471107
6. PICK1 inhibits the E3 ubiquitin ligase activity of Parkin and reduces its neuronal protective effect.  |  He, J., et al. 2018. Proc Natl Acad Sci U S A. 115: E7193-E7201. PMID: 29987020
7. The PICK1/TLR4 complex on microglia is involved in the regulation of LPS-induced sepsis-associated encephalopathy.  |  Wang, L., et al. 2021. Int Immunopharmacol. 100: 108116. PMID: 34500285
8. The adaptor protein PICK1 targets the sorting receptor SorLA.  |  Binkle, L., et al. 2022. Mol Brain. 15: 18. PMID: 35183222
9. Investigating the allosteric response of the PICK1 PDZ domain to different ligands with all-atom simulations.  |  Stevens, AO., et al. 2022. Protein Sci. 31: e4474. PMID: 36251217
10. PICK1 modulates glycolysis and angiogenesis of hypoxic endothelial cells by regulating iron homeostasis.  |  Cao, Y., et al. 2024. Mol Cell Biochem. 479: 1297-1312. PMID: 37368155

The preferred PICK1 antibody is PICK1 Antibody (D-10): sc-390479.