Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PIASy CRISPR/Cas9 KO Plasmid (h): sc-403125
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PIASy CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PIASy genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PIASy Antibody (C-11): sc-166706
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PIASy CRISPR/Cas9 KO Plasmid (h) | sc-403125 | 20 µg | $397.00 |
Overview
PIAS4 encodes PIASy, a SUMO E3 ligase that modulates protein SUMOylation to fine-tune transcriptional programs, DNA damage responses, and innate immune signaling. PIASy regulates the activity, localization, and stability of diverse transcription factors and signaling intermediates, linking SUMO-dependent control to pathways such as NF-κB, interferon responses, and stress-activated networks. Through these mechanisms, PIASy influences cell-cycle progression, apoptosis, and chromatin-associated processes that are frequently perturbed in disease biology. Altered PIAS4/PIASy function has been studied in the context of oncogenic signaling, inflammatory regulation, and genome maintenance phenotypes relevant to tumorigenesis and cellular transformation models.
PIASy CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PIAS4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PIAS4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PIAS4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PIASy protein expression.
This CRISPR knockout system enables efficient generation of PIAS4-deficient cell models for investigation of PIASy signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PIAS4 exon(s) critical for PIASy function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PIAS4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PIASy CRISPR/Cas9 KO Plasmid (h) and PIASy CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PIAS4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PIASy HDR Plasmid (h) and PIASy HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PIAS4 homology arms to support homology-directed repair at defined PIAS4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.