PIASx Antibody (D-12): sc-166494
- PIASx Antibody (D-12) is a mouse monoclonal IgG2a κ PIASx antibody, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 521-540 near the C-terminus of PIASx of human origin
- PIASx Antibody (D-12) is recommended for detection of PIASx of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine and porcine
- Anti-PIASx Antibody (D-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PIASx Antibody (D-12) for WB and IHC(P) applications. These reagents are now offered in bundles with PIASx Antibody (D-12) (see ordering information below).
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PIASx Antibody (D-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects PIASx protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). PIASx (D-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PIASx protein plays a crucial role in regulating cytokine signaling, particularly in the IL-6-type family of cytokines, which are essential for gene activation, cell proliferation, and differentiation. PIASx functions as a negative regulator of signal transducer and activator of transcription (Stat) proteins, which are activated during cytokine signaling and are responsible for mediating cellular response to these signals. PIASx modulation of Stat activity is vital for maintaining balanced cellular responses to cytokines, preventing excessive or prolonged signaling that could lead to pathological conditions. PIASx undergoes alternative splicing, resulting in two isoforms, PIASx-α and PIASx-β, which exhibit distinct C-terminal regions that may influence functional properties. A putative zinc-binding motif and highly acidic region in PIASx protein further underscore structural significance and potential interactions with other proteins, enhancing PIASx role in cellular signaling pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PIASx Antibody (D-12) References:
- Negative regulation of the JAK/STAT pathway. | Starr, R. and Hilton, DJ. 1999. Bioessays. 21: 47-52. PMID: 10070253
- ARIP3 (androgen receptor-interacting protein 3) and other PIAS (protein inhibitor of activated STAT) proteins differ in their ability to modulate steroid receptor-dependent transcriptional activation. | Kotaja, N., et al. 2000. Mol Endocrinol. 14: 1986-2000. PMID: 11117529
- Induction of apoptosis by protein inhibitor of activated Stat1 through c-Jun NH2-terminal kinase activation. | Liu, B. and Shuai, K. 2001. J Biol Chem. 276: 36624-31. PMID: 11451946
- Molecular cloning of APRF, a novel IFN-stimulated gene factor 3 p91-related transcription factor involved in the gp130-mediated signaling pathway. | Akira, S., et al. 1994. Cell. 77: 63-71. PMID: 7512451
- Stat3: a STAT family member activated by tyrosine phosphorylation in response to epidermal growth factor and interleukin-6. | Zhong, Z., et al. 1994. Science. 264: 95-8. PMID: 8140422
- Interleukin-6-type cytokine signalling through the gp130/Jak/STAT pathway. | Heinrich, PC., et al. 1998. Biochem J. 334 (Pt 2): 297-314. PMID: 9716487
- Inhibition of Stat1-mediated gene activation by PIAS1. | Liu, B., et al. 1998. Proc Natl Acad Sci U S A. 95: 10626-31. PMID: 9724754
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PIASx Antibody (D-12) | sc-166494 | 200 µg/ml | $322.00 | |||
PIASx Antibody (D-12): m-IgG Fc BP-HRP Bundle | sc-528981 | 200 µg Ab; 10 µg BP | $361.00 | |||
PIASx Antibody (D-12): m-IgGκ BP-HRP Bundle | sc-521631 | 200 µg Ab, 40 µg BP | $361.00 | |||
PIASx Antibody (D-12): m-IgG2a BP-HRP Bundle | sc-547223 | 200 µg Ab; 10 µg BP | $361.00 | |||
PIASx Antibody (D-12) AC | sc-166494 AC | 500 µg/ml, 25% agarose | $424.00 | |||
PIASx Antibody (D-12) HRP | sc-166494 HRP | 200 µg/ml | $322.00 | |||
PIASx Antibody (D-12) FITC | sc-166494 FITC | 200 µg/ml | $336.00 | |||
PIASx Antibody (D-12) PE | sc-166494 PE | 200 µg/ml | $349.00 | |||
PIASx Antibody (D-12) Alexa Fluor® 488 | sc-166494 AF488 | 200 µg/ml | $364.00 | |||
PIASx Antibody (D-12) Alexa Fluor® 546 | sc-166494 AF546 | 200 µg/ml | $364.00 | |||
PIASx Antibody (D-12) Alexa Fluor® 594 | sc-166494 AF594 | 200 µg/ml | $364.00 | |||
PIASx Antibody (D-12) Alexa Fluor® 647 | sc-166494 AF647 | 200 µg/ml | $364.00 | |||
PIASx Antibody (D-12) Alexa Fluor® 680 | sc-166494 AF680 | 200 µg/ml | $364.00 | |||
PIASx Antibody (D-12) Alexa Fluor® 790 | sc-166494 AF790 | 200 µg/ml | $364.00 | |||
PIASx (D-12) Neutralizing Peptide | sc-166494 P | 100 µg/0.5 ml | $69.00 |