PI-9 Antibody (7D8): sc-59983

PI-9 Antibody (7D8) is a mouse monoclonal IgG1 antibody that detects Proteinase Inhibitor-9 (PI-9) in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). PI-9 is a crucial member of the Ovalbumin family of serine proteinase inhibitors (serpins), primarily functioning to regulate serine proteinase activity, which is vital for maintaining cellular homeostasis and protecting against unwanted apoptosis. PI-9 is predominantly expressed in the placenta, lung, and cytotoxic lymphocytes, where PI-9 plays a significant role in inhibiting granzyme B, a serine protease that can induce apoptosis by promoting DNA degradation. PI-9′s ability to inhibit granzyme B is particularly important in the context of immune responses, as PI-9 helps to safeguard healthy cells from being targeted during cytotoxic attacks. Additionally, PI-9 is present in the nuclei of human cytotoxic cells, endothelial cells, and epithelial cells, indicating PI-9′s involvement in nuclear processes and suggesting a complex regulatory mechanism for nucleocytoplasmic distribution. PI-9 expression is rapidly induced by estrogen and upregulated in response to inflammatory stimuli, further highlighting PI-9′s role in cellular protection during immune challenges and potential implications in various human and murine tumors. With diverse applications and critical functions, anti-PI-9 antibody (7D8) is an essential tool for researchers studying apoptosis, immune regulation, and cancer biology.

The preferred PI-9 antibody is PI-9 Antibody (F-6): sc-390501.