PI 3-kinase p85α Antibody (B-9): sc-1637
- PI 3-kinase p85α Antibody (B-9) is a mouse monoclonal IgG1 κ PI 3-kinase p85α antibody, cited in 230 publications, provided at 200 µg/ml
- raised against amino acids 333-430 mapping within the N-terminus SH2 domain of PI 3-kinase p85α of PI 3-kinase of human origin
- PI 3-kinase p85 alpha Antibody (B-9) is recommended for detection of PI 3-kinase p85α of mouse, rat and human origin by WB, IP, IF and IHC(P)
- PI 3-kinase p85 alpha Antibody (B-9) is available conjugated to Alexa Fluor® 546 or Alexa Fluor® 594 for WB (RGB), IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for PI 3-kinase p85α Antibody (B-9) for WB and IHC(P) applications. These reagents are now offered in bundles with PI 3-kinase p85α Antibody (B-9) (see ordering information below).
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PI 3-kinase p85α Antibody (B-9) is a mouse monoclonal IgG1 kappa light chain antibody raised against amino acids 333-430 within the N-terminal SH2 domain of human PI 3-kinase p85α, with reactivity in mouse, rat, and human species. PI 3-kinase p85α monoclonal antibody (B-9) is validated for applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin embedded sections (IHCP). PI 3-kinase p85α, also known as PIK3R1 or phosphoinositide-3-kinase regulatory subunit 1, is a critical component of phosphatidylinositol 3-kinase (PI3K), which regulates key cellular processes such as growth, survival, metabolism, and motility. The p85α subunit serves as a regulatory adapter that couples the catalytic p110 subunit to activated protein tyrosine kinases, controlling the kinase′s activity and localization. PI3K′s activity is central to intracellular signaling pathways, including the AKT/mTOR pathway, which is vital for cell proliferation and survival. The p85α subunit contains SH2 and SH3 domains that mediate interactions with phosphorylated tyrosine residues on receptor tyrosine kinases and other signaling proteins, ensuring precise regulation of PI3K signaling. Dysregulation of PI3K activity, often through mutations or altered expression of p85α, is implicated in various cancers and metabolic disorders. PI 3-kinase p85α monoclonal antibody (B-9) provides a robust tool for studying PI 3-kinase p85α′s role in cellular signaling and its implications in health and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PI 3-kinase p85α Antibody (B-9) References:
- Phosphatidylinositol 3-kinase: structure and expression of the 110 kd catalytic subunit. | Hiles, ID., et al. 1992. Cell. 70: 419-29. PMID: 1322797
- Characterization of two 85 kd proteins that associate with receptor tyrosine kinases, middle-T/pp60c-src complexes, and PI3-kinase. | Otsu, M., et al. 1991. Cell. 65: 91-104. PMID: 1707345
- Cloning of PI3 kinase-associated p85 utilizing a novel method for expression/cloning of target proteins for receptor tyrosine kinases. | Skolnik, EY., et al. 1991. Cell. 65: 83-90. PMID: 1849461
- Cloning and characterization of a G protein-activated human phosphoinositide-3 kinase. | Stoyanov, B., et al. 1995. Science. 269: 690-3. PMID: 7624799
- Cloning of a novel, ubiquitously expressed human phosphatidylinositol 3-kinase and identification of its binding site on p85. | Hu, P., et al. 1993. Mol Cell Biol. 13: 7677-88. PMID: 8246984
- P110delta, a novel phosphoinositide 3-kinase in leukocytes. | Vanhaesebroeck, B., et al. 1997. Proc Natl Acad Sci U S A. 94: 4330-5. PMID: 9113989
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PI 3-kinase p85α Antibody (B-9) | sc-1637 | 200 µg/ml | $322.00 | |||
PI 3-kinase p85α Antibody (B-9): m-IgG Fc BP-HRP Bundle | sc-526468 | 200 µg Ab; 10 µg BP | $361.00 | |||
PI 3-kinase p85α Antibody (B-9): m-IgG1 BP-HRP Bundle | sc-531841 | 200 µg Ab; 20 µg BP | $361.00 | |||
PI 3-kinase p85α Antibody (B-9) Alexa Fluor® 546 | sc-1637 AF546 | 200 µg/ml | $364.00 | |||
PI 3-kinase p85α Antibody (B-9) Alexa Fluor® 594 | sc-1637 AF594 | 200 µg/ml | $364.00 | |||
PI 3-kinase p85α Antibody (B-9) Alexa Fluor® 680 | sc-1637 AF680 | 200 µg/ml | $364.00 | |||
PI 3-kinase p85α Antibody (B-9) Alexa Fluor® 790 | sc-1637 AF790 | 200 µg/ml | $364.00 |