Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PI 3-kinase p110γ CRISPR/Cas9 KO Plasmid (h): sc-401043
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PI 3-kinase p110γ CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PI 3-kinase p110γ genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PI 3-kinase p110γ Antibody (D-12): sc-166365
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PI 3-kinase p110γ CRISPR/Cas9 KO Plasmid (h) | sc-401043 | 20 µg | $397.00 |
Overview
PIK3CG encodes the catalytic p110γ subunit of class IB phosphoinositide 3-kinase, a key mediator of GPCR-driven phosphoinositide signaling in hematopoietic and other cell types. PI 3-kinase p110γ phosphorylates phosphatidylinositol lipids to generate PIP3, promoting AKT-dependent survival programs, cytoskeletal remodeling, and directed chemotaxis through downstream effectors such as PDK1, mTOR, and small GTPase regulators. This pathway integrates signals from chemokine receptors, complement receptors, and inflammatory mediators to control leukocyte activation, migration, and oxidative burst. Dysregulated PIK3CG signaling has been implicated in aberrant inflammatory responses and immune cell dysfunction, and it is also studied in the context of tumor–immune interactions and microenvironmental signaling.
PI 3-kinase p110γ CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PIK3CG gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PIK3CG together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PIK3CG open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PI 3-kinase p110γ protein expression.
This CRISPR knockout system enables efficient generation of PIK3CG-deficient cell models for investigation of PI 3-kinase p110γ signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PIK3CG exon(s) critical for PI 3-kinase p110γ function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PIK3CG genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PI 3-kinase p110γ CRISPR/Cas9 KO Plasmid (h) and PI 3-kinase p110γ CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PIK3CG locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PI 3-kinase p110γ HDR Plasmid (h) and PI 3-kinase p110γ HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PIK3CG homology arms to support homology-directed repair at defined PIK3CG target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.